LabCell™ Ham’s F12 Plates

INTENDED USE

LabCell™ Ham’s F12 is used for the culture of Chinese Hamster Ovarian Cells or other mammalian cells due to the addition of serum. The zinc content in Ham’s F12 is higher than Ham’s F10, which significantly improves the regeneration ability of CHO cells.

BacterLab performs quality control on each product batch.

Parameter	Standard	Result
Sterility	No bacterial growth after incubation at 35 – 37°C for 18 – 24 hours	Pass

For 1 liter of medium (reference)

	Ham’s F12	Ham’s F12.1 mg/l	Ham’s F12.12 mg/l
1	Glycine	7,51	7,51
	L-Alanine	9	9
	L-Arginine Monohydrochloride	211	211
	L-Asparagine Monohydrate	15,01	15,01
	L-Aspartic Acid	13,3	13,3
	L-Cysteine Monohydrochloride Monohydrate	35	35
	L-Glutamic Acid	14,7	14,7
	L-Glutamine	146	/
	L-Histidine Monohydrochloride Monohydrate	20,96	20,96
	L-Isoleucine	3,94	3,94
	L-Leucine	13,1	13,1
	L-Lysine Monohydrochloride	36,5	36,5
	L-Methionine	4,48	4,48
	L-Phenylalanine	4,96	4,96
	L-Proline	34,5	34,5
	L-Serine	10,5	10,5
	L-Threonine	11,9	11,9
	L-Tryptophan	2,04	2,04
	L-Tyrosine Disodium Salt Dihydrate	7,78	7,78
	L-Valine	11,7	11,7

2	Calcium Chloride Dihydrate	44,1	44,1
	Cupric Sulfate Pentahydrate	0,0025	0,0025
	Ferrous Sulfate Heptahydrate	0,834	0,834
	Magnesium Chloride Hexahydrate	123	123
	Potassium Chloride	224	224
	Sodium Bicarbonate	1176	1176
	Sodium Chloride	7599	7599
	Sodium Phosphate Dibasic Anhydrous	142,04	142,04
	Zinc Sulfate Heptahydrate	0,863	0,863

3	Choline Chloride	13,96	13,96
	D-Biotin	0,0073	0,0073
	D-Ca Pantothenate	0,48	0,48
	Folic Acid	1,32	1,32
	Myo-Inositol	18	18
	Nicotinamide	0,037	0,037
	Pyridoxine Hydrochloride	0,062	0,062
	Riboflavin	0,038	0,038
	Thiamine Hydrochloride	0,34	0,34
	Vitamin B12	1,36	1,36

4	D-Glucose Anhydrous	1802	1802
	Hypoxanthine	4,08	4,08
	Linoleic Acid	0,084	0,084
	Phenol Red Solution Salt	1,3	1,3
	Putrescine + 2HCL	0,161	0,161
	Sodium Pyruvate	110	110
	Thioctic Acid	0,21	0,21
	Thymidine	0,73	0,73

pH of the ready-to-use medium at 25⁰C: 7,3 ± 0,3

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
LabCell™ Ham’s F12	13005	500mL/ bottle or as per customer request.