Pollution sources in cleanroom

What are the sources of clean room contamination ?

Clean rooms are designed to meet certain standards for cleanliness of fecal particles according to levels appropriate to user needs. However, sources of pollution for clean rooms can spread particulates throughout the environment, affecting research efficiency, manufactured products, etc. Therefore, it is necessary to understand the sources of pollution . cleanrooms are popular so that further steps can be taken to ensure that the ideal state of the cleanroom environment is maintained .

1. Staff:

The most common source of contamination in cleanrooms is the people working in the cleanroom. When humans shed dead cells from their bodies, they become a source of microorganisms that cause pollution in the environment. In addition, hair and fibers on employee clothes can also be sources of clean room pollution.

Employees working in clean rooms need to comply with dress regulations.

Picture 1: Seminar on the assessment and control of cleanroom systems

2. Water source:

  • Water is used during operations in clean rooms, so if it is not filtered through a process that ensures all sources of contamination are eliminated and is tested regularly, water can be a source of very dangerous microorganisms.
  • In addition, water streaks in clean rooms, if not cleaned properly, will be an environment for microorganisms to grow and spread.
  • Regularly check the water source used and ensure proper cleanliness of the room.

3. Equipment – supplies:

  • Clean room equipment such as biological safety cabinets and analytical balances, although designed and calculated to meet the needs of use as well as the cleanliness level of the room, can also become a source of infection if not properly cleaned and maintained.
  • Materials used in clean rooms, if not strictly controlled, will become a source of microbiological infection when brought into the room of use.
  • Periodically check and maintain equipment in the clean room, control contamination of materials entering the clean room.

4. Office accessories:

  • Although the possibility is quite small, employees bringing pencils and pens into the room also poses a risk of contamination.
  • There are separate office accessories for use in the clean room, with specific regulations on the location of use that all employees need to comply with.


The above causes of pollution are for reference only and do not include all causes of clean room pollution . Depending on different usage needs such as using clean rooms for research, testing or production ,… which pollutants in clean rooms will be different. It is necessary to check and monitor each specific clean room situation to determine the source of infection, thereby building the most effective staff management and clean room cleaning mechanism.

Learn about microbiological testing method: https://labone.vn/phuong-phap-kiem-tra-vi-sinh-cho-phong-sach/

Clean room microbiological testing products:

  • TraceMedia™ Sabouraud Dextrose Agar (SDA) : Ready-to-use medium on 90mm petri dishes for fungal culture.


  • TraceMedia™ Tryptone Soy Agar : Ready-to-use medium on 90mm petri dishes for non-selective cultivation of easily grown bacteria.


  • SwabCollect™ Neutralizing Buffer

The neutral buffer neutralizes the bactericidal and bacteriostatic effects of chlorine and quaternary ammonium detergents.


  • SwabCollect™ Buffered Peptone Water

Pre-enrichment medium for Salmonella spp. and CroNobacter sakazakii: from processed food sources.




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