BacterChrom™ Orientation Agar Plates

Ready-to-use chromogenic medium on 90mm plates for isolating and differentiating urinary tract pathogenic microorganisms.
Code: 01010
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ Orientation Agar is a non-selective chromogenic culture medium intended for use in the qualitative direct detection, differentiation and presumptive identification of uropathogens to aid in the diagnosis of urine tract infections. The test is performed with urine samples. Results can be interpreted after 18-24 h of aerobic incubation at 35-37 °C. A lack of growth or the absence of colonies on BacterChrom™ Orientation Agar does not preclude the presence of bacteria.
BacterChrom™ Orientation Agar is not intended to diagnose infection nor to guide nor monitor treatment for infections.
BacterChrom™ Orientation Agar can also be used with veterinary samples
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ Orientation Agar utilizes a combination of chromogenic substrates and selective agents to differentiate and identify pathogens based on the specific enzymatic activity of the bacteria. Each species or group of species produces distinct colony colors, allowing rapid and accurate identification directly from urine samples. Selective agents suppress the growth of non-target organisms, improving specificity.

BacterChrom™ Orientation Agar can be used with the following specimens:
- In clinical field: urine
- In veterinary field: veterinary samples
Sampling and transport equipment must be used in accordance with the recommendations of their suppliers for the conservation of strains.
Relevant samples can be processed either by direct inoculation onto plates
Bring the agar plates to room temperature. Dry the agar plates in an incubator by partially opening the agar plates
Streak sample onto plate.
Incubate in aerobic conditions at 35 – 37 °C for 18 – 24 hours

RESULTS

Microorganisms	Typical colony appearance
Gram (-)
Klebsiella, Enterobacter, Citrobacter, Serratia	dark pink to reddish
MSSA	metallic blue (+/- reddish halo)
Proteus, Morganella, Providencia	brown halo
Proteus vulgaris	blue with brown halo
Pseudomonas	translucent ( +/- natural pigmentation cream to green)
Acinetobacter	cream
Stenotrophomonas	Colorless
Gram (+)
S. agalactiae	light blue
Enterococcus	turquoise blue
S. aureus	golden, opaque, small
S. epidermidis	cream, pinpoint colonies
S. saprophyticus	pink, opaque, small
Yeasts
Candida albicans	cream, pinpoint colonies

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Strains	Incubation Conditions	Culture results
E. coli ATCC 35218	Incubate at 35 – 37°C for 18 – 24 hours	Good growth, dark pink to red colonies
S. aureus ATCC 25923		Good growth, white colonies with natural pigment
E. faecalis ATCC 29212		Good growth, blue colonies