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INTENDED USE
- BacterTube™ LIM Broth is a liquid medium recommended for use in qualitative procedures for the isolation of Group B Streptococcus (GBS) from clinical specimens containing mixed bacterial flora.
PRINCIPLES
- Peptones, heart infusion, and yeast extract supply nitrogenous compounds and vitamins essential for bacterial growth. Dextrose is a source of carbon and energy. Sodium chloride provides essential electrolytes and maintains osmotic equilibrium. Disodium phosphate and sodium carbonate are buffers that counteract the acidity produced during the fermentation of dextrose and protect the hemolysin from inactivation by the acid. Colistin and nalidixic acid are selective agents that inhibit the growth of gram-negative bacteria.
- Obtain a single swab or two separate swabs of the distal vagina and anorectum between 35-37 weeks gestation.
- Inoculate LIM Broth with the swab or both swabs together.
- Incubate the tube aerobically or in 5% CO₂ at 33 – 37°C for 18 – 24 hours.
- After incubation, subculture LIM Broth to a sheep blood agar plate (nonselective) and incubate aerobically or in 5% CO₂ at 33 – 37°C for 18 – 24 hours.
RESULTS
- Examine the blood agar plate at 24 and 48 hours for large, gray, translucent colonies with a small zone of beta-hemolysis or no hemolysis
- Perform a catalase test and Gram stain to verify that colonies are catalase-negative, gram-positive cocci.
- Definitive identification of Group B Streptococcus requires additional biochemical and/or serological testing.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Staphylococcus agalactiae ATCC 12386 | 18 – 24 hours, 33 – 37°C, 5% CO2 | Good growth |
| Enterococcus faecalis ATCC 29212 | Good growth | |
| Eschirichia coli ATCC 25922 | Partially to completely inhibited |
For 1 liter of medium
| Beef Heart Infusion | 3,1 g |
| Casein Peptone | 20,0 g |
| Yeast Extract | 10,0 g |
| Glucose | 2,0 g |
| Sodium Chloride | 2,0 g |
| Disodium Phosphate | 0,4 g |
| Sodium Carbonate | 2,5 g |
| Colistin Sulfate | 0,01 g |
| Nalidixic Acid | 0,015 g |
pH of the ready-to-use medium at 25°C: 7,8 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ LIM Broth | 08018 | 50 tubes/ box or as per customer request. |
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