BacterPlate™ BHI Agar with Chloramphenicol
- Ready-to-use medium on 90mm plates for culturing easily cultivated bacteria, yeasts, and molds.
- Code: 05055
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ BHI Agar with Chloramphenicol is recommended for the cultivation of fastidious pathogenic bacteria, yeasts and moulds from clinical and non clinical samples.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterPlate™ BHI Agar with Chloramphenicol The medium contains brain and heart infusions that provide essential nutrients for microbial growth. Peptone and dextrose supply additional nitrogen, carbon, and energy sources. Sodium chloride maintains osmotic balance, while agar serves as the solidifying agent. The rich composition makes it ideal for the growth of a wide variety of organisms, including those that are nutritionally demanding. Chloramphenicol inhibits bacterial growth, creating favorable conditions for fungal growth.
- Streak the surface of the medium with a bacterial suspension or inoculate directly from the sample. For fungal cultures, use appropriate inoculation techniques.
- Incubate at the temperature and atmospheric conditions appropriate for the organism being cultured (e.g., aerobic, anaerobic, or microaerophilic).
- Typical incubation: 35 – 37°C for 18 – 48 hours for bacteria; up to 7 days for fungal cultures.
RESULTS
- Observe the growth characteristics, including colony morphology, color, and size.
- Perform further biochemical or molecular tests for identification if needed.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Strains | Incubation Conditions | Culture results |
| S.aureus ATCC 25923 | Incubate at 35 – 37°C for 48 hours | Good growth |
| E. coli ATCC 35218 | Inhibition, no growth | |
| C.albicans ATCC 10231 | Good growth |
For 1 liter of medium
| Brain heart extract | 17,5 g |
| Pancreatic digest of gelatin | 10,0 g |
| Sodium chloride | 5,0 g |
| Disodium phosphate | 2,5 g |
| Glucose | 2,0 g |
| Chloramphenicol | 0,1 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 250C: 7,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ BHI Agar with Chloramphenicol | 05055 | 10 plates/ box or as per customer request. |
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