Vn
INTENDED USE
- BacterPlate™ R2A Agar is a medium destined for the enumeration of total viable aerobic microorganisms in treated water such as purified water, highly purified water and water for injectable preparation.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Present in small quantity, the nutritive elements permit the development of stressed microorganisms. The combination of mineral salts maintains the osmotic balance. The presence of sodium pyruvate activates the bacterial metabolism.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Aseptically filter through a membrane a known volume of the sample to test.
- Deposit the membrane on the surface of the agar, filtered side up and making sure that the membrane and agar are in close contact.
- Incubate at 30 – 35 °C for at least 5 days.
INTENDED USE
- Enumerate the plates containing less than 150 CFU per membrane filter.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| P. aeruginosa ATCC 9027 | 30–35°C for 48 hours | Good growth |
For 1 liter of medium
| Yeast extract | 0,5 g |
| Proteose peptone | 0,5 g |
| Acid hydrolysate of casein | 0,5 g |
| Glucose | 0,5 g |
| Starch | 0,5 g |
| Dipotassium phosphate | 0,3 g |
| Magnesium sulfate, anhydrous | 0,024 g |
| Sodium pyruvate | 0,3 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ R2A Agar | 05057 | 10 plates/ box or as per customer request. |
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