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BacterContact™ DG 18 + Chloramphenicol
- Ready-to-use medium on 60mm plates for surface microbiological sampling with the addition of a neutralizing agent to deactivate the surface disinfectant
- Code: 12006
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
INTENDED USE
- BacterContact™ DG 18 + Chloramphenicol for surface microbiological sampling with the addition of a neutralizing agent to deactivate the surface disinfectant.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterContact™ DG 18 + Chloramphenicol: It is a medium supplemented with 18% Glycerol to reduce the water activity from around 0.99 to 0.95 and contains chloramphenicol to inhibit bacterial growth. The medium also contains dichloran, which prevents the rapid spread of slime molds and limits the colony size of other genera, thereby reducing the colony count.
- Prepare the surface to be tested: Clean the surface to be tested using a 70% alcohol solution or another cleaning solution. Then, wait for the surface to dry completely.
- Open the pre-packaged BacterContact plates: Ensure that the packaging of the plates is not torn or damaged before opening.
- Place the Contact plate on the surface to be tested: Press the Rodac plate onto the surface to be tested. The recommended contact time between the plate and the test surface is 10 seconds with a pressing force of 500g.
- Seal the Rodac plate: Make sure that the lid of the Rodac plate is tightly closed. Wipe the surface again with 70% alcohol.
- Evaluation of Results: Incubate under the following conditions
- 30 – 35 °C for 72 ± 6 hours (NF EN ISO 21149, NF EN ISO 18415)
- 20 – 25 °C for 3 to 5 days for Total Microbial Count (Pharmacopoeia)
RESULTS
- After incubation for the required period, usually from 3 to 5 days, the plates are examined for the presence of fungi. The results are evaluated by counting the number of fungal colonies on the plates
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Microorganisms | Incubation conditions | Expected results |
| C. albicans ATCC 10231 | 24 – 48 hours of incubation at 30 – 35°C | PR ≥ 50 % |
| S. cerevisiae ATCC 20827 | ||
| A. brasiliensis ATCC 16404 | 72 hours of incubation at 20 – 25°C | PR ≥ 70 % |
For 1 liter of medium
| Tryptone | 5,0 g |
| Glucose | 10,0 g |
| Monopotassium phosphate | 1,0 g |
| Magnesium sulfate, H2O | 0,5 g |
| Dichloran (dichloro-2,6-nitro-4-aniline) | 2,0 mg |
| Chloramphenicol | 0,1 g |
| Glycerol | 220,0 g |
| Agar | 15,0 g |
pH of the ready-to-use media at 25 °C: 5,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterContact™ DG 18 + Chloramphenicol | 12006 | 10 plates/ box or as per customer request. |
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