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SpongesCollect™ Cellulose Neutralizing Buffer, 10 mL
- A microbiological sampling tool for surfaces, using a cellulose sponge pre-moistened with Neutralizing Buffer to neutralize the effects of residual sanitizing agents and protect microorganisms during transport and analysis.
- Code: 4107009
- Packaging: 100 pieces/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
SpongesCollect™ Cellulose Neutralizing Buffer, 10 mL is designed to collect microorganisms from surfaces in food processing, healthcare, and pharmaceutical environments. The primary purpose of this product is to assist in collecting microbial samples from surfaces, equipment, and surrounding areas in food production facilities. Neutralizing Buffer helps neutralize the effects of residual sanitizing agents on surfaces, ensuring that microorganisms are not affected during transport and analysis.
SpongesCollect™ Cellulose Neutralizing Buffer, 10 mL uses a Cellulose sponge combined with 10 mL of Neutralizing Buffer collect microorganisms from surfaces. The Neutralizing Buffer neutralizes residual sanitizing agents, such as chlorine, quaternary ammonium compounds, iodine, and phenolic, protecting microorganisms from the effects of these substances. However, unlike nutrient media, Neutralizing Buffer does not provide nutrients for microorganism growth. Therefore, this solution only serves to neutralize and does not support microbial growth, helping maintain the viability of microorganisms throughout transport without altering the sample’s characteristics.
- Inspect the SpongesCollect™ product to ensure there is no damage or contamination during storage.
- Write sample information on the provided label attached to the product packaging.
- Tear the bag along the pre-marked perforated line.
- Push the sponge near the edge of the bag so that part of the sponge is exposed for easy handling. Hold the sponge from the outside of the bag and carefully remove it directly by hand, making sure not to touch the inside surface of the bag to avoid contamination.
- Immerse the sponge into a sterile buffer/culture medium as required (if necessary).
- Rub the SpongesCollect™ sponge on the area to be sampled.
- After sampling, return the sponge to the bag. Ensure the sponge does not fall out and avoid direct contact with the outer surface of the packaging.
- Use the provided tie to seal the SpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag.
- The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.
| Growth Promotion Test: ≤ 103 CFU/mL microorganisms | ||
| Control strain | Incubation Conditions | Specifications |
| S.aureus ATCC 6538 | 24 – 48 h at 35 – 37°C | Good growth |
| Sterility control | No bacterial growth after incubation at 35 – 37°C for 72 hrs | |
| Appearance | The solution contains a homogeneous sponges, without any stratification, light amber, clear. | |
Tween 80 (Polysorbate 80)…………………………………. 3,0 mL
Histidine………………………………………………………….. 0,1 g
Sodium thiosulfate……………………………………………. 0.8 g
Soy lecithin……………………………………………………… 0,3 g
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
* Adjusted and /or supplemented as required to meet performance criteria
| Product Name | Code | Packaging |
| SpongesCollect™ Cellulose Neutralizing Buffer, 10 mL | 4107009 | 100 pieces/box or customer request. |
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