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SpongesCollect™ Polyurethane Letheen Broth, 10 mL
- Microbial Sampling Tool for surface sampling, using a polyurethane sponge moistened with Letheen Broth to neutralize the effects of disinfectants and protect microorganisms during transport and analysis.
- Code: 4107004
- Packaging: 100 pieces/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
SpongesCollect™ Polyurethane Letheen Broth, 10mL is designed for microbiological sampling from surfaces in food processing, healthcare, and pharmaceutical environments. The primary purpose of this product is to support the collection of microbial samples from large, easily accessible surfaces and surrounding areas within food production facilities. Letheen Broth is a nutrient-rich pre-enrichment medium that helps test the effectiveness of disinfection processes while maintaining the viability and stability of microorganisms during transport and analysis.
SpongesCollect™ Polyurethane Letheen Broth, 10mL uses a biocide-free polyurethane sponge combined with 10 mL of Letheen Broth to collect microorganisms from surfaces. Letheen Broth is a nutrient-rich medium containing lecithin and Tween 80. Lecithin neutralizes quaternary ammonium compounds, a key component in disinfectants, ensuring that microorganisms are not affected by residual disinfectants on surfaces. Tween 80 neutralizes disinfectants such as phenol, hexachlorophene, and formalin, protecting microorganisms from their harmful effects. This process helps recover and maintain the stability of microorganisms in food processing environments, ensuring they grow well throughout transport and analysis before being transferred to selective media without any alteration of their characteristics.
- Inspect the SpongesCollect™ product to ensure there is no damage or contamination during storage.
- Write sample information on the provided label attached to the product packaging.
- Tear the bag along the pre-marked perforated line.
- Push the sponge near the edge of the bag so that part of the sponge is exposed for easy handling. Hold the sponge from the outside of the bag and carefully remove it directly by hand, making sure not to touch the inside surface of the bag to avoid contamination.
- Immerse the sponge into a sterile buffer/culture medium as required (if necessary).
- Rub the SpongesCollect™ sponge on the area to be sampled.
- After sampling, return the sponge to the bag. Ensure the sponge does not fall out and avoid direct contact with the outer surface of the packaging.
- Use the provided tie to seal the SpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag.
- The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.
| Growth Promotion Test: ≤ 103 CFU/mL microorganisms | ||
| Control strain | Incubation Conditions | Specifications |
| E. coli ATCC 25922 | 24 – 48 h at 35 – 37°C | Good growth |
| S. aureus ATCC 25923 | 24 – 48 h at 35 – 37°C | Good growth |
| S. typhimurium ATCC 14028 | 24 – 48 h at 35 – 37°C | Good growth |
| Sterility control | No bacterial growth after incubation at 35 – 37°C for 72 hrs | |
| Appearance | The solution contains a homogeneous sponges, without any stratification, light yellow, clear. | |
Casein peptone…………………………………………………. 5,0 g
Beef extract……………………………………………………… 5,0 g
Polysorbate 80 (Tween 80)…………………………………. 5,0 g
Lecithin…………………………………………………………… 0,7 g
Meat peptone……………………………………………………. 20,0 g
Sodium bisulfite……………………………………………….. 0,1 g
Sodium chloride……………………………………………….. 5,0 g
Yeast extract……………………………………………………. 2,0 g
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
* Adjusted and /or supplemented as required to meet performance criteria
| Product Name | Code | Packaging |
| SpongesCollect™ Polyurethane Letheen Broth, 10 mL | 4107004 | 100 pieces/box or customer request. |
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