StickSpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL

Microbial Sampling Tool for surface sampling, using a plastic stick with an attached Cellulose sponge impregnated with Buffered Peptone Water medium to recover and protect microorganisms during transportation and analysis.
Code: 4107028
Packaging: 100 pieces/box or customer request.
Storage Conditions: 4 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

StickSpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL is intended for microbiological monitoring of surfaces and environmental objects, as well as for confirming and evaluating the effectiveness of sterilization and disinfection processes. The product facilitates the collection of bacterial samples from surfaces, especially from hard-to-reach areas such as drains, equipment, and surrounding areas in food manufacturing facilities. It is suitable for use in medical, pharmaceutical, cosmetic, food, and other hygiene-sensitive industries

PRINCIPLES

StickSpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL utilizes a plastic stick with an attached cellulose sponge combined with 10 mL of Buffered Peptone Water containing neutralizer to collect microorganisms from surfaces. The Buffered Peptone Water with neutralizing agents effectively neutralizes residual disinfectants and sanitizers on the sampled surfaces, supporting the recovery and preservation of microorganisms during sample collection, transport, and analysis. The sponge material and moisture content ensure optimal microbial viability and stability.

Inspect the StickSpongesCollect™ product to ensure there is no damage or contamination during storage.
Write sample information on the provided label attached to the product packaging.
Wear the provided sterile gloves (if available).
Tear the bag along the pre-marked perforated line.
Remove the StickSpongesCollect™ stick by pulling the bag from the outside. Be careful not to touch the inside of the bag.
Rub the StickSpongesCollect™ sponge on the area to be sampled.
After sampling, return the sampling stick to the bag, making sure not to insert it too deep to avoid touching the inside of the bag.
Remove the sponge from the stick by holding the sponge from the outside of the bag and gently shaking until the sponge comes off the stick.
Use the provided tie to seal the StickSpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag..
The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.

Growth Promotion Test: ≤ 10³ CFU/mL microorganisms
Control strain	Incubation Conditions	Specifications
S. typhimurium ATCC 14028	18 – 24 h at 35 – 37°C	Good growth
S.aureus ATCC 25923	18 – 24 h at 35 – 37°C	Good growth
E. coli ATCC 25922	18 – 24 h at 35 – 37°C	Good growth
Sterility control	No bacterial growth after incubation at 35 – 37°C for 72 hrs
Appearance	The solution contains a homogeneous sponges, without any stratification, light yellow, clear.

Bacteriological peptone……………………………………… 10,0 g

Sodium Choloride……………………………………………… 5,0 g

Sodium phosphate dibasic 12-hydrate………………….. 9,0 g

Potassium phosphate monobasic anhydrous………….. 1,5 g

Tween 80 (Polysorbate 80)…………………………………. 3 mL

Histidine………………………………………………………….. 0,1 g

Sodium thiosulfate…………………………………………….. 0,8 g

Soy lecithin………………………………………………………. 0,3 g

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

* Adjusted and /or supplemented as required to meet performance criteria

Product Name	Code	Packaging
StickSpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL	4107028	100 pieces/box or customer request.