Vn
BacterBagTM Dry Alkaline Saline Peptone Water (ASPW)
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. Media used for the growth and enrichment of Vibrio bacteria.
- Code: 10024
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
INTENDED USE
BacterBag™ Dry Alkaline Saline Peptone Water (ASPW) is a high-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag, designed to simplify the preparation steps in microbiological culturing processes. Each BacterBag™ Dry Alkaline Saline Peptone Water (ASPW), when supplemented with sterile distilled water, produces 20L of complete medium, ready to use without complex preparation
This medium used as a primary and secondary enrichment medium to detect Vibrio spp. in human and animal food products as well as environmental samples.
Typical ingredients meet food microbiological standards NF EN ISO 21872-1.
Key Features:
- Convenient: Simply add purified water, and the medium is ready to use in minutes.
- Cost-effective: Lightweight and easy to transport, no need for weighing or sterilizing, reducing the workload of the medium preparation department.
- Safe: No need for handling glassware or measuring the medium; used bags can be disposed of with regular waste.
Quality assurance: Each batch is quality-certified, reducing the need for quality control checks
PRINCIPLES
BacterBag™ Dry Alkaline Saline Peptone Water (ASPW) was first developed by Shread, Donovan and Lee3 as a non-selective liquid enrichment medium for the cultivation of Aeromonas species. Cruickshank reported that increased media pH could be used effectively in culturing Vibrio species that are tolerant to high alkalinity and chloride.
BacterBag™ Dry Alkaline Saline Peptone Water (ASPW) complies with ISO/TS 21872-1:2007, which specifies a horizontal method for the detection of two major pathogenic Vibrio species that cause enteric disease in humans: V. parahaemolyticus and V. cholera. This method is applicable to products intended for human consumption and animal feed, as well as environmental samples in the food production and processing sector. Peptone provides carbon, nitrogen and essential nutrients to organisms. High sodium chloride concentrations, in addition to maintaining osmotic balance, also have the effect of inhibiting the accompanying microflora.
- Add Xg sample to 9XmL or 9Xg Alkaline Saline Peptone Water medium at ambient temperature.
- Incubate for about 6 ± 1 hour, at 41.5 ± 1°C for fresh samples or 37 ± 1°C for frozen or salted samples.
- Take 1mL of the incubated solution and inoculate it with 10ml of Alkaline Saline Peptone Water and incubate for about 18 ± 1 hour at 37 ± 1°C.
- From the obtained culture fluid, take 1 loop of culture rod and inoculate it onto the surface of TCBS Agar medium and a second selective isolation medium to obtain characteristic colonies.
- Incubate the inoculated plate at 37°C for about 24 ± 3 hours (or a suitable second selective medium).
- Identify characteristic colonies of pathogenic Vibrio species present on the plate. Select 5 types of colonies to culture on Saline Nutrient Agar medium for biochemical determination.
RESULT INTERPRETATION
- This medium is used for the recovery and enrichment of bacteria present in the sample. Thus, secondary culturing in other selective media is necessary for bacterial identification
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| V. furnissii DSM 14383 | 41,5°C for 18 – 24 hours | Good growth |
| Vibrio parahaemolyticus ATCC 17802 (WDCM 00185b) | Good growth |
For 1 liter of medium (reference)
| Peptone | 20,0 g |
| Sodium chloride | 20,0 g |
pH of the ready-to-use medium at 25°C: 8,6 ± 0,2
| Product Name | Code | Packaging |
| BacterBagTM Dry Alkaline Saline Peptone Water (ASPW) | 10024 | Equivalent to 20L of medium per bag or as per customer requirements. |
Related products
BacterTube™ Lowen Stein Jensen (LJ)
- The selective medium used for culturing and isolating Mycobacterium species.
- Code: 08043
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterBag™ Lactose Broth
- The medium used for the detection of Enterobacteriaceae particularly coliform and Salmonella species from water, food and dairy products as per Standard Methods and FDA BAM.
- Code: 10003
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterTube™ Sterile Physiological Saline (NS 0,85%)
- Solution used for sample dilution and preparation of bacterial suspensions for identification and antibiotic susceptibility testing
- Code: 08019
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterBagTM Dry Sterile Physiological Saline
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. Sample dilution solution, bacterial suspension used for identification and antibiogram.
- Code: 10023
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterBag™ Tryptone Soya Broth (TSB)
- A highly nutritious general purpose medium for the cultivation of a wide variety of micro-organisms. For identification of Salmonella species from food samples in accordance with FDA BAM.
- Code: 10007
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterBagTM Dry Lactose Broth
- High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. Specifically designed to detection of Enterobacteriaceae particularly coliform and Salmonella species from water, food and dairy products as per Standard Methods and FDA BAM.
- Code: 10015
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterTube™ McFarland 0.5 Turbidity Standard
- Ready-to-use liquid standard used for bacterial suspension standardization in antimicrobial susceptibility testing.
- Code: 08024
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterTube™ PBS Buffer 1X
- Buffered saline solutions are commonly used in biological research and microbiological testing.
- Code: 08025
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterTube™ Peptone Water
- Microbial enrichment medium or as a basic carbohydrate fermentation medium.
- Code: 08040
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
BacterBag™ Maximum Recovery Diluent
- Protective and isotonic medium used for maximal recovery of microorganisms from a variety of sources.
- Code: 10004
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.