LabCell™ Basal Medium Eagle (BME)

INTENDED USE

LabCell™ Basal Medium Eagle (BME) is a widely used synthetic basal medium to support the proliferation of cell lines (Hela, L-Cells) and primary cells (fibroblasts).

BacterLab performs quality control on each product batch.

Parameter	Standard	Result
Sterility	No bacterial growth after incubation at 35 – 37°C for 18 – 24 hours	Pass

For 1 liter of medium (reference)

Component	Concentration (mg/L)
Amino Acids
L-Arginine hydrochloride	21.0
L-Cystine 2HCl	16.0
L-Histidine	8.0
L-Isoleucine	26.0
L-Leucine	26.0
L-Lysine hydrochloride	36.47
L-Methionine	7.5
L-Phenylalanine	16.5
L-Threonine	24.0
L-Tryptophan	4.0
L-Tyrosine disodium salt dihydrate	26.0
L-Valine	23.5
Vitamins
Biotin	1.0
Choline chloride	1.0
D-Calcium pantothenate	1.0
Folic Acid	1.0
Nicotinamide	1.0
Pyridoxal hydrochloride	1.0
Riboflavin	0.1
Thiamine hydrochloride	1.0
i-Inositol	2.0
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	200.0
Magnesium Sulfate (MgSO4) (anhyd.)	97.7
Potassium Chloride (KCl)	400.0
Sodium Bicarbonate (NaHCO3)	2200.0
Sodium Chloride (NaCl)	6800.0
Sodium Phosphate monobasic (NaH2PO4-H2O)	140.0
Other ingredients
D-Glucose (Dextrose)	1000.0
Phenol Red	10.0