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SwabCollect™ D/E Neutralizing Broth, 1 mL
- Microbial Sampling Tool for surface sampling, using a swab and a plastic tube containing D/E Neutralizing Broth to recover and protect microorganisms, during transport and analysis.
- Code: 4108029
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.
INTENDED USE
SwabCollect™ D/E Neutralizing Broth, 1 mL is designed for microbiological sampling from surfaces in food processing, healthcare, and pharmaceutical environments. The primary purpose of this product is to support the collection of microbial samples from large, easily accessible surfaces and surrounding areas within food production facilities. D/E Neutralizing Broth helps neutralize the effects of disinfectants and protects microorganisms throughout transport and analysis.
PRINCIPLES
SwabCollect™ D/E Neutralizing Broth, 1 mL combines a sterile swab with a plastic tube containing 1 mL of D/E Neutralizing Broth to collect microorganisms from surfaces. D/E Neutralizing Broth neutralizes the effects of disinfectants such as chlorine, quaternary ammonium compounds, iodine, phenolic compounds, mercury, formaldehyde, and glutaraldehyde, protecting microorganisms from their harmful effects. This medium provides the necessary nutrients to recover and promote microorganism growth, while maintaining their viability during transport. D/E Neutralizing Broth not only neutralizes disinfectants but also preserves the integrity of microorganisms, ensuring accurate analysis results. Bromocresol purple is included in the medium as a pH indicator, allowing monitoring of the neutralization process.
- Inspect the SwabCollect™ product to ensure it is not damaged or contaminated during storage.
- Write the sample information on the pre-labeled sticker on the plastic tube containing the solution.
- Carefully open the cap of the solution tube to avoid contaminating the internal environment.
- Immerse the swab tip into the solution in the tube, ensuring the swab tip is completely moistened with the solution.
- Collect the sample by gently rubbing the swab on the area to be sampled. Be careful not to touch the swab tip with your hands.
- Insert the swab back into the tube containing the solution.
- Break off the excess swab tip to fit the tube.
- Close the plastic tube tightly to protect the sample and prevent contamination during transport.
- Transport the sample immediately to the laboratory to ensure the stability of the microorganisms, following sample storage guidelines.
- SwabCollect™ D/E Neutralizing Broth, 1 mL is produced in a closed environment, adhering to strict hygiene regulations. The plastic tube and swab are sterilized using gamma irradiation, ensuring no microorganisms affect the quality of the sample.
BacterLab ensures the quality of each product batch of the medium by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Escherichia coli ATCC 25922 (WDCM 00013) | 35 – 37°C for 24 – 48 hours | Good growth, yellow solution |
| Staphylococcus aureus ATCC 25923 (WDCM 00034) | Good growth, yellow solution | |
| Salmonella typhimurium ATCC 14028 (WDCM 00031) | Good growth, yellow solution |
Tools
- Sterile swab
- Sterile plastic tube, size 16 x 100 mm
Product Name Code Packaging SwabCollect™ Alkaline Saline Peptone Water, 1 mL 4108021 50 set/box or customer request Each kit includes: one plastic tube with sample transport medium and one sterile swab.
Medium
- Volume of medium in the plastic tube: 1 mL
- Components of the medium:
For 1 liter of medium
| Dextrose | 10,0 g |
| Sodium bisulfite | 2,5 g |
| Lecithin | 7,0 g |
| Yeast extract | 2,5 g |
| Sodium thiosulfate | 6,0 g |
| Sodium thioglycollate | 1,0 g |
| Casein peptone | 5,0 g |
| Brom cresol purple | 0,02 g |
| Polysorbate 80 (Tween 80) | 5,0 g |
pH of the ready-to-use medium at 25°C: 7,6 ± 0,2
| Product Name | Code | Packaging |
| SwabCollect™ D/E Neutralizing Broth, 1 mL | 4108029 | 50 set/box or customer request |
| Each kit includes: one plastic tube with sample transport medium and one sterile swab. |
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