BacterChrom™ C3GR Plates
- Ready-to-use chromogenic medium on 90mm plates for the direct qualitative detection of third-generation cephalosporin-resistant Enterobacteria (C3GR -E).
- Code: 01027
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ C3GR is a selective and differential chromogenic medium intended for the qualitative direct detection of gastrointestinal colonization with third-generation cephalosporin-resistant Enterobacteria (C3GR-E). This medium can also serve as an early warning indicator for diagnostic tests, signaling the possible presence of multidrug-resistant bacteria.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ C3GR employs chromogenic substrates that interact with specific enzymatic activities unique to C3GR-E, producing distinct colony colors for visual identification. The selective agents incorporated into the medium inhibit non-target organisms, ensuring high specificity. This combination facilitates rapid presumptive identification of resistant Enterobacteria, supporting infection control efforts in clinical environments.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate at 35 – 37 °C for 18 – 24 hours in aerobic conditions
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance | 
| C3GR E.coli | dark pink to reddish | 
| C3GR KEC (Klebsiella, Enterobacter, Citrobacter) | metallic blue (+/- reddish halo) | 
| C3GR Proteus | brown halo | 
| Gram (+) strains | inhibited | 
| Non Resistant Other Gram (-) strains | inhibited | 
| Yeasts | mostly inhibited | 
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results | 
| C3GR E. coli CIP 103982 | Incubate for 18 – 24 hours at 35 – 37 °C | reddish, small colonies | 
| C3GR K. pneumoniae ATCC 700603 | metallic blue (+/- reddish halo) colonies | |
| E. faecalis ATCC 29212 | inhibited | |
| P. aeruginosa ATCC 9027 | inhibited | |
| E. coli ATCC 35218 | inhibited | |
| C. albicans ATCC 10231 | inhibited | |
| S. aureus ATCC 25923 | inhibited | 
For 1 liter of medium
| Peptone and yeast extract | 17,0 g | 
| Chromogenic mix | 27,3 g | 
| Selective mix | 0,37 g | 
| Agar | 15,0 g | 
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging | 
| BacterChrom™ C3GR | 01027 | 10 plates/box or as per customer request. | 
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