BacterChrom™ LIN-R Plates
- Ready-to-use chromogenic medium on 90mm plates for detecting and differentiating linezolid-resistant Gram (+) bacteria.
- Code: 01026
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ LIN-R is a selective and differential chromogenic medium designed for the detection, isolation, and differentiation of Gram-positive bacteria resistant to linezolid (LIN-R). It is particularly intended for screening specimens such as nasal, rectal, and perianal swabs, as well as stool samples. The medium supports monitoring programs for antibiotic resistance, aiding in infection control efforts in healthcare environments
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ LIN-R incorporates chromogenic substrates that react with specific enzymatic activities of linezolid-resistant Gram-positive bacteria, producing distinctive colony colors. Selective agents in the medium inhibit the growth of non-target organisms, ensuring specificity. The medium offers a rapid and efficient method for presumptive identification of resistant bacteria, simplifying laboratory workflows.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate at 35 – 37 °C for 36 – 48 hours in aerobic conditions.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| LZD S.aureus | pink |
| LZD S.epidermidis | pink |
| LZD Enterococcus | steel blue |
| Other Gram-positive LZD bacteria | limited growth or inhibited |
| Gram-negative bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC and NCTC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. epidermidis LZD NCTC 13924 | Incubate for 36 – 48 hours at 35 – 37 °C | Pink with pink halo colonies |
| E. faecalis LZD NCTC 14360 | Steel blue with blue halo colonies | |
| S. aureus ATCC 25923 | Inhibited | |
| E. faecalis ATCC 29212 | Inhibited | |
| E. coli ATCC35218 | Inhibited |
For 1 liter of medium
| Peptone and yeast extract | 20,0 g |
| Salt | 7,0 g |
| Chromogenic mix and Selective mix | 0,4 g |
| Growth factors | 8 mL |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 6,9 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ LIN-R | 01026 | 10 plates/box or as per customer request. |
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