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BacterTube™ Thioglycollate Broth
- Ready-to-use liquid medium used for the cultivation and isolation of obligate and facultative anaerobic and microaerophilic bacteria.
- Code: 08017
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Thioglycollate Broth is designed for the cultivation and isolation of obligate and facultative anaerobic and microaerophilic bacteria. It is also suitable for aseptic process simulations in pharmaceutical and clinical laboratories.
PRINCIPLES
- This medium contains peptone from casein and yeast extract, which provide essential nutrients for bacterial growth. D(+)-Glucose serves as an energy source. Sodium thioglycollate and L-cystine act as reducing agents, creating an anaerobic environment by lowering the oxidation-reduction potential. This environment supports the growth of anaerobes and prevents the accumulation of peroxides, which can be harmful to certain microorganisms. The sulfhydryl groups in these reducing agents can inactivate heavy metals, making the medium suitable for samples containing such compounds.
- Allow the medium tube to stabilize at room temperature.
- Using aseptic techniques, inoculate the medium with the sample material, ensuring the sample reaches the bottom of the bottle.
- To maintain anaerobic conditions, overlay the medium with a 1 cm layer of sterile liquid paraffin or agar solution if necessary.
- Incubate at 30 – 35°C for several days, depending on the growth requirements of the target organisms.
RESULTS
| Microoraganisms | Signs of organism growth |
| Candida albicans | Flocculated Growth |
| Clostridium | Growth and/or opaque colonies |
| Peptostreptococcus (anaerobic) | Growth and/or opaque colonies |
| Clostridium perfringens | Growth and/or opaque colonies |
| Bacteroides fragilis | Growth and/or opaque colonies |
| Bacteroides vulgatus | Growth and/or opaque colonies |
| Staphylococcus aureus subsp. aureus | Growth and/or opaque colonies |
| Pseudomonas aeruginosa | Growth and/or opaque colonies |
| Micrococcus luteus | Growth and/or opaque colonies |
| Streptococcus pneumoniae (Pneumococcus) | Growth and/or opaque colonies |
| Escherichia coli | Growth and/or opaque colonies |
| Salmonella typhimurium | Growth and/or opaque colonies |
| Salmonella (general) | Growth and/or opaque colonies |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Clostridium perfringens ATCC 13124 | 24 – 72 hours, 35 – 37°C | Good growth |
| Staphylococcus aureus ATCC 25923 | Good growth | |
| Pseudomonas aeruginosa ATCC 27853 | Good growth | |
| Eschirichia coli ATCC 25922 | Good growth |
For 1 liter of medium
| Peptone from Casein | 15,0 g |
| Yeast extract | 5,0 g |
| Dextrose (Glucose) | 5,5 g |
| Sodium chloride | 2,5 g |
| L-Cystine | 0,5 g |
| Sodium thioglycollate | 0,5 g |
pH of the ready-to-use medium at 25°C: 7,1 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Thioglycollate Broth | 08017 | 50 tubes/ box or as per customer request. |
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