BacterTube™ Fluorocult

Enrichment for the simultaneous detection of total coliforms and E.coli in water, food and dairy products by the fluorogenic procedure.
Code: 08048
Packaging: 50 tubes/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

BacterTube™ Fluorocult Enrichment for the simultaneous detection of total coliforms and E.coli in water, food and dairy products by the fluorogenic procedure.

PRINCIPLES

BacterTube™ Fluorocult contains phosphate buffer to guarantee a high growth rate for total coliforms. Lauryl sulfate inhibits most of the accompanying Gram-positive bacteria. By adding the chromogenic substrate 5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside, which is cleaved by coliforms, and the fluorogenic substrate 4-methylumbelliferyl-ß-D-glucuronide, which is highly specific for E. coli, both total coliforms and E. coli can be detected simultaneously. The color change of the medium from yellow to blue indicates the presence of coliforms. Additionally, blue fluorescence under long-wavelength ultraviolet light enables rapid detection of E. coli. When tryptophan is added to the broth, the indole reaction is easily performed by adding Kocavs reagent. The formation of a red ring further confirms the presence of E. coli. The enzyme synthesis process is amplified by 1-isopropyl-ß-D-1-thio-galactopyranoside, which increases the activity of ß-D-galactosidase.

Incubation: 18 – 24 hours at a temperature of 36 ± 1.0°C under aerobic conditions. If incubated at room temperature (+20 to +25°C), the incubation time may extend up to 48 hours.
Observe and record the results

RESULTS

Coliforms: The color of the broth changes to blue.
coli: The broth turns blue and exhibits blue fluorescence under long-wave UV light (366 nm). Adding Kovacs reagent for the indole test results in a red ring, which also confirms the presence of E. coli.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	The medium turns blue	Fluorescence	Indole reaction
Culture conditions: 18 – 24 hours, incubated at 35 – 37°C_, inoculum ≤ 10² CFU/mL, aerobic
Escherichia coli ATCC 25922	+	+	+
Klebsiella pneumoniae ATCC 31488	+	–	–
Pseudomonas aeruginosa ATCC 10145	+	–	–

For 1 liter of medium

Tryptose	5,0 g
Sodium Chloride	5,0 g
Sorbitol	1,0 g
Tryptophan	1,0 g
Dipotassium hydrogen phosphate	2,7 g
Potassium dihydrogen phosphate	2,0 g
Lauryl sulfate sodium salt	0,1 g
5-bromo-4-chloro- 3-indolyl-ß-D-galactopyranoside (X-GAL)	0,08 g
4-methylum- belliferyl-ß-D-glucuronide (MUG)	0,05 g
1-isopropyl-ß-D-1- thio-galactopyranoside (IPTG)	0,1 g