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INTENDED USE
- BacterTube™ Nitrate Broth is used to detect the ability of bacteria to reduce nitrate.
PRINCIPLES
- BacterTube™ Nitrate Broth is a basic medium that includes potassium nitrate, which is reduced by the nitroreductase enzyme of microorganisms. Some microorganisms reduce nitrate to nitrite, while others reduce nitrate to NH₃, N₂, etc. The reduction of nitrate to nitrite is detected by adding sulfanilic acid (reagent A) and N,N-dimethyl-1-naphthylamine (reagent B). Sulfanilic acid reacts with nitrite to form a diazonium salt, which then combines with N,N-dimethyl-1-naphthylamine to form a red-colored complex. Other forms of nitrate reduction products (NH₃, N₂, etc.) may not show the red color change, which can be confirmed by adding zinc powder (reagent C).
- Prior to inoculation, allow medium to equilibrate to room temperature
- Use a sterile inoculation loop to transfer a sample from an isolated colony on the agar plate into the test tube containing the medium.
- Incubate the tube at 35°C, Aerobic for 18 – 48 hours.
- Examine the tube after 18 – 24 hours and again after 42 – 48 hours of incubation and gas production in the Durham tube.
- Observe and record the results.
RESULTS
- Positive nitrate reduction: A red color appears after adding reagents A and B to the test tube, or no color change occurs after adding zinc powder.
- Negative nitrate reduction: No color change occurs after adding reagents A and B, and a red color appears after adding zinc powder.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Acinetobacter baumannii ATCC 19606 | 18 – 24 hours, 35 – 37oC | Negative nitrate reduction
|
| Escherichia coli ATCC 25922 | Positive nitrate reduction |
For 1 liter of medium
| Peptone | 5,0 g |
| Beef extract | 3,0 g |
| Potassium nitrate | 1,0 g |
| Distilled Water | 1000 mL |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Nitrate Broth | 08047 | 50 tubes/ box or as per customer request. |
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