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BacterBag™ Buffered Peptone Water (BPW)
- The non-selective enrichment medium is used in the pre-enrichment step of the Salmonella testing process.
- Code: 10001
- Packaging: 5 liter/ bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
INTENDED USE
- BacterBag™ Buffered Peptone Water (BPW) is a general-purpose diluent used for various activities and standards, including sample preparation, preparation of stock suspensions, and serial dilutions. It is also used for the pre-enrichment of Salmonella by allowing the recovery of microorganisms subjected to sublethal processing methods, such as spray drying, pasteurization, preservative actions, high osmotic pressure, and high acidity. Additionally, it is used as a suspension and recovery medium for the quantification of Listeria monocytogenes.
PRINCIPLES
- BacterBag™ Buffered Peptone Water (BPW) Peptone, serving as a source of carbon, nitrogen, and vitamins essential for bacterial growth and development. Disodium phosphate, anhydrous, and Monopotassium phosphate to prevent bacterial damage caused by pH fluctuations in the medium. Chloride, which creates a saline environment to maintain the internal and external pH balance of bacteria.
1. Preparation of stock solution:
- Add 10 or 25g of the product to be analyzed into a volumetric flask containing 90 or 225mL of BacterBag™ Buffered Peptone Water (BPW).
- Homogenize thoroughly using an appropriate homogenizer.
- Specifically for Salmonella or Enterobacteria enrichment, incubate while strictly following the appropriate analysis procedure.
2. Preparation of serial dilutions:
- Add 1 mL of the stock suspension to a tube containing 9 mL of BacterBag™ Buffered Peptone Water (BPW).
- Mix thoroughly.
- Repeat as necessary to achieve the desired dilution level.
RESULTS
4.1. Preparation of stock solution:
- Add 10 or 25g of the product to be analyzed into a volumetric flask containing 90 or 225mL of BacterBag™ Buffered Peptone Water (BPW).
- Homogenize thoroughly using an appropriate homogenizer.
- Specifically for Salmonella or Enterobacteria enrichment, incubate while strictly following the appropriate analysis procedure.
4.2. Preparation of serial dilutions:
- Add 1 mL of the stock suspension to a tube containing 9 mL of BacterBag™ Buffered Peptone Water (BPW).
- Mix thoroughly.
- Repeat as necessary to achieve the desired dilution level.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. Typhimurium ATCC 14028 |
18 – 24 hours at 35 – 37°C |
Good growth |
| E. coli ATCC 25922 | Good growth | |
| S. aureus ATCC 25923 | Good growth |
For 1L of medium (reference):
| Peptone | 10,0 g |
| Sodium Choloride | 5,0 g |
| Disodium phosphate, anhydrous, | 3,57 g |
| Monopotassium phosphate, anhydrous , | 1,5 g |
pH of the complete medium at 25°C: 7,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterBag™ Buffered Peptone Water (BPW) | 10001 | 5 liter/ bag or as per customer requirements. |
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