BacterBag™ Tryptone Soya Broth (TSB)

A highly nutritious general purpose medium for the cultivation of a wide variety of micro-organisms. For identification of Salmonella species from food samples in accordance with FDA BAM.
Code: 10007
Packaging: 5 liter/ bag or as per customer requirements.
Storage Conditions: 8 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 06 months from the date of manufacture.

PURPOSE OF USE

BacterBag™ Tryptone Soya Broth (TSB) for highly nutritious general purpose medium for the cultivation of a wide variety of micro-organisms. The medium is primarily intended for the culture of fungi and aerobe bacteria. For identification of Salmonella species from food samples in accordance with FDA BAM.

PRINCIPLE

BacterBag™ Tryptone Soya Broth (TSB) contains Tryptone, Tryptose, Soybean Peptone and Yeast Extract to provide carbon, nitrogen compounds, long-chain amino acids, vitamins and other trace mineral sources necessary for the growth of microorganisms. Dextrose provides the necessary carbon source for the medium. Sodium chloride maintains the osmotic balance of the medium. Dipotassium hydrogen phosphate acts as a buffer.

Contamination check

Control sterility by inoculating samples into water prepared as above so that – no dilution exceeds 1:10.
Incubate for 14 days at 20 – 25ºC.

Testing Salmonella in food samples

Add 25g of the food sample(s) suspected to be contaminated with Salmonella into 225mL culture broth (1:9 ratio).
Incubate at 35 ± 2 º C for 24 ± 2 hours in accordance with the BAM protocol.
The incubated sample is processed for isolation of the species by inoculation into selective media such as Selenite broth, Rappaport Vassiliadis Medium.
Incubation for 24hrs at appropriate temperatures.

BacterLab ensures the quality of each product batch by testing it with standard ATCC strains.

Reference strains	Incubation conditions	Expected results
Staphylococcus aureus ATCC 25923 (WDCM 00034)	18 – 24 hours at 35 – 37°C	Good growth
Escherichia coli ATCC 25922 (WDCM 00013)		Good growth
Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)		Good growth
Salmonella Typhimurium ATCC 14028 (WDCM 00031)		Good growth
No culturing	72 hours at 35 – 37°C	No contamination