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BacterPlate™ SDA with Chloramphenicol
- Ready-to-use medium on 90mm plates for selective fungal cultivation. Applied for the selective isolation of fungi from clinical specimens.
- Code: 05028
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ SDA with Chloramphenicol is recommended for the isolation of yeasts and molds, especially when the samples are highly contaminated with bacteria.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Peptic digest of Meat is the nitrogen source for growth. Glucose is an energy source.
- Chloramphenicol is a heat-stable, broad spectrum antibiotic which inhibits the development of contaminating microflora.
- The acid pH favors the growth of yeasts and molds.
- Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
- Inoculate test samples from the enrichment medium onto the surface of the agar plates.
- Incubate the inoculated plates under aerobic conditions at 25 – 30°C for 72 – 96 hours.
RESULTS
- Separately enumerate yeast colonies and molds
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference Strains | Incubation Conditions | Expected results |
| C. albicans ATCC 10231 | 30°C, 3 – 5 days | ≥ 50 % |
| A. brasiliensis ATCC 16404 | ≥ 50 % | |
| S. cerevisiae ATCC 20827 | ≥ 50 % | |
| E. coli ATCC 35218 | Inhibited |
For 1 liter of medium
| Mycological peptone | 10,0 g |
| Glucose | 40,0 g |
| Pancreatic digest of casein | 5,0 g |
| Chloramphenicol | 0,008 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 5,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterPlate™ SDA with Chloramphenicol | 05028 | 10 plates/ box or as per customer request. |
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