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BacterPlate™ Plate Count Agar (PCA)
- Ready-to-use medium on 90mm plates for counting colony. Applied to determine the total number of aerobic bacteria.
- Code: 05001
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 04 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Plate Count Agar (PCA) containing glucose and yeast extract is used in food bacteriology to enumerate aerobic bacteria in milk, meats, meat-based products, other food products, as well as for the analysis of pharmaceuticals, cosmetics and their associated raw materials.
- It is also used for the enumeration psychrotrophic microorganisms.
- The typical composition corresponds to that defined in the standards NF EN ISO 4833-1 & 2, NF ISO 17410, XP V08-034; T90-425 and ISO 14461-1.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLE
- The nutrients supplied by Tryptone, vitamins from yeast extract, and glucose used as energy source favor the growth of most bacteria. In dairy bacteriology, it is recommended to add 1 g of powdered skim milk per liter of reconstituted media (PCA with Skimmed Milk)
Surface inoculation (NF EN ISO 4833-2):
- Dry the plates in an incubator with the covers partially removed.
- Transfer 0,1 mL of the sample to test to the surface of the BacterPlate™ Plate Count Agar (PCA) along with its serial dilutions.
- Inoculate the plate by using a sterile triangle.
- Incubate at 30 ± 1°C for 72 ± 3 hours.
NOTE:
- A spiral inoculation system can also be used.
- For the detection of psychrotrophic bacteria in foods (NF ISO 17410), incubate the plates for 10 days at 6,5 °C.
RESULTS
- Count plates containing less than 300 colonies
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| E. coli ATCC 35218 | Incubate for 48 – 72 hours at 35 – 37 °C | PR ≥ 70% |
| S. aureus ATCC 25923 | PR ≥ 70% |
For 1 liter of medium
| Tryptone | 5,0 g |
| Yeast extract | 2,5 g |
| Glucose | 1,0 g |
| Agar | 12,0 g |
pH of the ready-to-use medium at 25°C: 7,0 ± 0,2
*Please note that this composition is for reference purposes. Additionally, Our company will prepare the components as required or according to the tender documents.
| Product Name | Code | Packaging |
| BacterPlate™ Plate Count Agar (PCA) | 05001 | 10 plates/ box or as per customer request. |
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