BacterPlate™ Two-Compartment Medium MRSA / ESBL

Ready-to-use medium on 90mm plates for the detection and differentiation of Methicillin-resistant Staphylococcus aureus (MRSA) and ESBL producing bacteria
Code: 05049
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ Two-Compartment Medium MRSA / ESBL is a ready-to-use two-compartment agar medium designed for the detection and differentiation of Methicillin-resistant Staphylococcus aureus (MRSA) and Extended-Spectrum Beta-Lactamase (ESBL) producing Gram-negative bacteria. This product is used in microbiological research, clinical diagnostics, and microbiological quality control of food, water, pharmaceuticals, and animal-derived biological samples.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterPlate™ Two-Compartment Medium MRSA / ESBL:

MRSA Compartment: This is a selective medium containing bile salts, cefoxitin, or oxacillin, which helps isolate Methicillin-resistant Staphylococcus aureus (MRSA). The medium is accompanied by a color indicator that helps identify characteristic colonies (usually green/purple/red depending on the medium composition).
ESBL Compartment: This medium contains selective components such as cefotaxime or ceftazidime, which are used to isolate Enterobacteriaceae strains that produce ESBL. A color indicator is also included to differentiate between ESBL (+) and ESBL (-) colonies.

Allow the agar plates to reach room temperature before use.
Dry the agar plates in an incubator with the lid slightly open.
Inoculate the sample by spreading it evenly on the surface of the agar using a sterile inoculating loop.
Incubate at 35–37°C for 18–24 hours.

RESULTS

MRSA Compartment

MRSA (+): Colonies have a characteristic color (usually pink/purple/red), growing well.
MRSA (–): No growth or weak growth. (MSSA)

ESBL Compartment

ESBL (+): Colonies have a characteristic color, growing well despite the presence of antibiotics.
ESBL (–): No growth or weak growth.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
MRSA Compartment
S. aureus ATCC 43300	Incubate at 35–37°C for 18–24 hours.	Good growth, mauve colonies
S. aureus ATCC 25923	Incubate at 35–37°C for 18–24 hours.	Weak growth to no growth.
ESBL Compartment
ESBL E.coli CIP 103982	Incubate at 35–37°C for 18–24 hours.	Good growth, small reddish colonies
ESBL K. Pneumoniae ATCC 700603		Good growth, blue colonies
E.faecalis ATCC 29212		Inhibited