BacterPlate™ BAYK Agar

Ready-to-use medium on 90mm plates for the non-selective culture of anaerobic bacteria.
Code: 05034
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterPlate™ BAYK Agar is a specialized nutrient medium designed for the isolation and qualitative cultivation of anaerobic microorganisms, including difficult-to-culture and slow-growing strains.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterPlate™ BAYK Agar is formulated by incorporating defibrinated sheep or horse blood into a Columbia agar base, creating a nutrient-rich environment for the growth of anaerobic microorganisms. The blood components provide essential growth factors and nutrients, enhancing the recovery of fastidious and slow-growing anaerobic bacteria while enabling the observation of hemolytic reactions.
The medium’s robust nutrient profile and reduced oxygen conditions support the isolation and cultivation of anaerobic microorganisms, facilitating their detection, identification, and characterization. This makes BAYK Agar an indispensable tool in clinical microbiology for understanding infection processes and diagnosing anaerobic-related clinical conditions.

Allow the agar plates to reach room temperature. Dry the plates in an incubator by slightly opening the lid.
Inoculate test samples from the enrichment medium onto the surface of the agar plates.
Incubate the inoculated plates under aerobic conditions at 35 – 37°C with 5 – 10% CO₂for 18 – 24 hours.

RESULTS

After incubation for the required duration, observe the colonies growing on the surface of the plate for characteristic traits such as colony size, color, and morphology.
Identification of the isolated bacteria must be carried out through appropriate subsequent tests

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation Conditions	Standard (P_R)
Bacteroides fragilis ATCC 25285	Incubate for 18 – 24 hours, at 35 – 37°C, 5 – 10 % CO₂	Good growth
Bacteroides melaninogenicus ATCC 25611		Good growth
Peptostreptococcus anaerobius ATCC 27337		Good growth

For 1 liter of medium