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BacterBag™ Dry Half Fraser Broth
- Dry powder form of Half Fraser Broth medium, used for the enrichment of Listeria monocytogenes.
- Code: 10014
- Packaging: Equivalent to 20L of medium per bag or as per customer requirements.
- Storage Conditions: 8 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 36 months from the date of manufacture.
INTENDED USE
- BacterBag™ Dry Half Fraser Broth is a high-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag, designed to simplify the preparation steps in microbiological culturing processes. Each BacterBag™ Dry Half Fraser Broth, when supplemented with sterile distilled water, produces 20L of complete medium, ready to use without complex preparation.
- This medium supports the non-selective enrichment of Listeria monocytogenes, providing conditions for the bacteria to recover and multiply before being transferred to selective culture media, improving the detection and recovery of Listeria from test samples.
- Key Features:
-
- Convenient: Simply add purified water, and the medium is ready to use in minutes.
- Cost-effective: Lightweight and easy to transport, no need for weighing or sterilizing, reducing the workload of the medium preparation department.
- Safe: No need for handling glassware or measuring the medium; used bags can be disposed of with regular waste.
- Quality assurance: Each batch is quality-certified, reducing the need for quality control checks.
PRINCIPLES
- BacterBag™ Dry Half Fraser Broth contains nutrients such as peptones, glucose, and yeast extract, providing nitrogen, carbon, and growth factors for bacteria. Additives like Tween 80, phosphate, and sodium help maintain stable pH and osmotic pressure, creating an ideal environment for bacterial recovery and multiplication before transferring to selective culture media.
- Add the appropriate amount of sterile distilled water into the BacterBag™ Dry Half Fraser Broth packet according to the ratio provided on the packaging to create a complete solution..
- Homogenize until the medium is completely dissolved.
- Take 25 g of the sample to be analyzed and add it to a volumetric flask, then add 90 or 225 mL of the rehydrated BacterBag™ Dry Half Fraser Broth
- Mix the contents thoroughly using an appropriate homogenizer.
- Incubate the prepared culture at 35 – 37ºC for 18 – 24 hours.
RESULTS
- This medium is used to recover and enrich bacteria in samples. After incubation, secondary culturing should be performed in other selective media to identify the bacteria.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| L. monocytogenes ATCC 13932 | 35 – 37°C for 18 – 24 hours | Good growth |
| E. coli ATCC 35218 | No growth, inhibited |
For 1 liter of medium
| Peptones | 20,0 g |
| Yeast extract | 5,0 g |
| Glucose | 20,0 g |
| Tween 80 | 1,08 g |
| Dipotassium phosphate | 2,0 g |
| Sodium | 5,0 g |
| Ammonium citrate | 2,0 g |
| Magnesium sulfate | 0,2 g |
| Manganese sulfate | 0,05g |
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterBag™ Dry Half Fraser Broth | 10014 | Equivalent to 20L of medium per bag or as per customer requirements. |
USER MANUAL_BacterBag™Dry Half Fraser Broth
MSDS_BacterBag™ Dry Half Fraser Broth
CoA_BacterBag™ Dry Half Fraser Broth
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