BacterBagTM Dry Tryptone Soya Broth (TSB)

High-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag. A highly nutritious general purpose medium for the cultivation of a wide variety of micro-organisms. For identification of Salmonella species from food samples in accordance with FDA BAM.
Code: 10019
Packaging: 5 liter/ bag or as per customer requirements.
Storage Conditions: 8 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 06 months from the date of manufacture.

INTENDED USE

BacterBag™ Dry Tryptone Soya Broth (TSB) is a high-quality dry medium, packaged in a transparent, lightweight, and convenient plastic bag, designed to simplify the preparation steps in microbiological culturing processes. Each BacterBag™ Dry Tryptone Soya Broth (TSB), when supplemented with sterile distilled water, produces 20L of complete medium, ready to use without complex preparation

This medium for highly nutritious general purpose medium for the cultivation of a wide variety of micro-organisms. The medium is primarily intended for the culture of fungi and aerobe bacteria. For identification of Salmonella species from food samples in accordance with FDA BAM.

Key Features:

Convenient: Simply add purified water, and the medium is ready to use in minutes.
Cost-effective: Lightweight and easy to transport, no need for weighing or sterilizing, reducing the workload of the medium preparation department.
Safe: No need for handling glassware or measuring the medium; used bags can be disposed of with regular waste.

Quality assurance: Each batch is quality-certified, reducing the need for quality control checks

PRINCIPLES

BacterBag™ Dry Tryptone Soya Broth (TSB) contains Tryptone, Tryptose, Soybean Peptone and Yeast Extract to provide carbon, nitrogen compounds, long-chain amino acids, vitamins and other trace mineral sources necessary for the growth of microorganisms. Dextrose provides the necessary carbon source for the medium. Sodium chloride maintains the osmotic balance of the medium. Dipotassium hydrogen phosphate acts as a buffer.

Contamination check

Control sterility by inoculating samples into water prepared as above so that – no dilution exceeds 1:10.
Incubate for 14 days at 20 – 25ºC.

Testing Salmonella in food samples

Add 25g of the food sample(s) suspected to be contaminated with Salmonella into 225mL culture broth (1:9 ratio).
Incubate at 35 ± 2 º C for 24 ± 2 hours in accordance with the BAM protocol.
The incubated sample is processed for isolation of the species by inoculation into selective media such as Selenite broth, Rappaport Vassiliadis Medium.
Incubation for 24hrs at appropriate temperatures.
Thoroughly mix and streak a 3 mm loopful of the incubated broth on Bismuth Sulphite Agar, XLD agar and Hektoen Enteric Agar.
Organism is identified by its colony characteristics in respective media.
The organism can be confirmed through biochemical and serological tests.

RESULT INTERPRETATION

This medium is used for the recovery and enrichment of bacteria present in the sample. Thus, secondary culturing in other selective media is necessary for bacterial identification

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Staphylococcus aureus ATCC 25923 (WDCM 00034)	18 – 24 hours at 35 – 37°C	Good growth
Escherichia coli ATCC 25922 (WDCM 00013)		Good growth
Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)		Good growth
Salmonella Typhimurium ATCC 14028 (WDCM 00031)		Good growth
No culturing	72 hours at 35 – 37°C	No contamination

For 1 liter of medium

Tryptone	8,5 g
Soya peptone	1,5 g
Sodium chloride	5,1 g
Dextrose (Glucose)	1,77 g
Dipotassium hydrogen phosphate	1,25 g
Tryptose	10,38 g
Yeast extract	3,0 g

pH of the ready-to-use medium at 25°C: 7,2 ± 0,2

Product Name	Code	Packaging
BacterBag^TM Dry Tryptone Soya Broth (TSB)	10019	Equivalent to 20L of medium per bag or as per customer requirements.