BacterChrom™ B.cepacia Plates
- Ready-to-use chromogenic medium on 90mm plates for the direct qualitative detection and differentiation of Burkholderia cepacia
- Code: 01023
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ B.cepacia is a selective chromogenic medium designed for the qualitative direct detection, differentiation, and presumptive identification of Burkholderia cepacia complex (BCC) bacteria.
- Additionally, BacterChrom™ B.cepacia can be applied in the pharmaceutical industry to analyze non-sterile, water-based products like creams or syrups for BCC contamination
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ B.cepacia employs chromogenic substrates that react with specific enzymatic activities of Burkholderia cepacia complex bacteria, resulting in distinct colony colors. The selective agents incorporated into the medium inhibit non-target organisms, ensuring high specificity and minimizing interference. This combination of chromogenic and selective properties facilitates the rapid presumptive identification of BCC bacteria.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Clinical field: Streak sample. Incubate at 35°C to 37°C during 36 to 72 h in aerobic atmosphere.
- Pharma industry: Streak sample or place the inoculated membrane (if using filtration) onto plate. Incubate at 30°C to 35°C during 36 to 72 h in aerobic atmosphere
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Burkholderia | green-blue +/- blue halo |
| Yeasts and mold | Limited growth or inhibited |
| Gram (+) bacteria | Inhibited |
| Gram (-) bacteria | Limited growth or inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| B. cepacia ATCC 25416 | Incubate for 36 – 72 hours at 35 – 37 °C | blue +/- blue halo colonies |
| S. epidermidis ATCC 12228 | Inhibited | |
| K. pneumoniae NCTC 13442 | Inhibited | |
| P. aeruginosa ATCC 9027 | Inhibited |
For 1 liter of medium
| Peptone | 11,0 g |
| Chromogenic mix and selective mix | 0,3 g |
| Agar | 15,0 g |
| Growth factor | 6,6 g |
pH of the ready-to-use medium at 25°C: 6,2 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ B.cepacia | 01023 | 10 plates/box or as per customer request. |
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