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BacterChrom™ B. cereus Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for detection and enumeration of Bacillus cereus group.
- Code: 01031
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChromTM B. cereus is a selective and differential culture medium designed for the detection and enumeration of Bacillus cereus group bacteria in environmental and food samples. It is intended for use in food safety testing and environmental monitoring to identify contamination with toxin-producing Bacillus cereus.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Bacillus cereus is a spore-forming bacterium commonly found in soil and various food products. It produces toxins that can cause two types of foodborne illnesses:
- Diarrheal illness: Associated with meat or vegetable-containing foods, occurring after long incubation periods (8 – 16 hours).
- Emetic illness: Associated with improperly stored cooked rice, occurring after short incubation periods (1 – 6 hours).
- This medium is formulated with selective agents to inhibit the growth of non-target organisms and chromogenic substrates for the differentiation of Bacillus cereus. The bacterium’s ability to hydrolyze specific substrates results in characteristic colony coloration, enabling rapid identification
- Allow plates to reach room temperature before inoculation
- Streak or spread the specimen directly onto the agar surface for isolation
- Incubate in aerobic conditions at 35 – 37 °C for 18 – 24 hours.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microoragnism | Typical colony appearance |
| Bacillus of cereus group | blue, with white halo |
| Other Bacillus | blue, colourless or inhibited |
| Gram negative bacteria | inhibited |
| Yeast and moulds | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| PERFORMANCE | RESULTS |
| Culture conditions: 18 – 24 hours, incubated at 35 – 37°C, inoculum ≤ 103 – 104 CFU/mL | |
| B. cereus ATCC 10876 | Blue with white halo |
| E. coli ATCC 35218 | Inhibited, no growth |
For 1 liter of medium
| Peptone and yeast extract | 8,0 g |
| Chromogenic mix | 0,3 g |
| Sodium chloride | 10,0 g |
| Selective mix | 0,3 g |
| Agar | 15,0 g |
pH of the ready-to-use medium at 25°C: 6,8 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ B. cereus Agar | 01031 | 10 plates/box or as per customer request. |
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