Vn
BacterChrom™ C. perfringens Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection and enumeration of Clostridium perfringens
- Code: 01032
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ C.perfringens is a selective chromogenic medium designed for the detection and enumeration of Clostridium perfringens in food, water, and environmental samples.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ C.perfringens utilizes chromogenic substrates that react with specific enzymatic activities of Clostridium perfringens, resulting in distinct orange colonies. Selective agents inhibit the growth of non-target organisms, ensuring specificity. This medium allows rapid and easy differentiation of C. perfringens from other microorganisms, streamlining microbiological workflows.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate in aerobic conditions at 37°C for 24 hours.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| C. perfringens | orange |
| Other bacteria | blue or inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| C. perfringens ATCC 12920 | 18 – 24 hours, 35 – 37°C | Orange colonies |
| E. faecalis ATCC 29212 | inhibited | |
| E. coli ATCC 35218 | inhibited |
For 1 liter of medium
| Peptone and yeast extract | 25,0 g |
| Chromogenic and selective mix | 1,4 g |
| Salt | 6,0 g |
| Agar | 15,0 g |
| Growth factors | 3,5 g |
| Selective mix | 2,0 g |
pH of the ready-to-use medium at 25°C: 7,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ C.perfringens | 01032 | 10 plates/box or as per customer request. |
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