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BacterChrom™ E.coli Plates
- Ready-to-use chromogenic medium on 90mm plates for the detection and enumeration of E. coli
- Code: 01040
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ E.coli is a selective chromogenic medium designed for the detecton and enumeraton of ß-glucuronidase positve E. coli in food, water and environmental samples.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ E.coli utilizes chromogenic substrates that react with specific enzymatic activities of E. coli, producing blue colonies for easy differentiation. Selective agents are incorporated to suppress non-target organisms, ensuring specificity. This formulation allows for the rapid and reliable detection of ß-glucuronidase-positive E. coli, streamlining microbiological workflows in food safety and environmental testing.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Streak the sample or place the inoculated membranes on plate surface.
- Incubate in aerobic conditions at 37°C for 24 hours.
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| E.coli | blue |
| Other Gram (-) bacteria | colorless |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| E. coli ATCC 35218 | Incubate for 24 hours at 35 – 37 °C | Blue colonies |
| S. aureus ATCC 25923 | Inhibited | |
| E. faecalis ATCC 29212 | Inhibited |
For 1 liter of medium
| Peptone and yeast | 8,3 g |
| Chromogenic mix | 9,0 g |
| Agar | 15,0 g |
| Sodium chloride | 5,0 g |
pH of the ready-to-use medium at 25°C: 6,0 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ E.coli | 01040 | 10 plates/box or as per customer request. |
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