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BacterChrom™ Staph aureus Agar
- Ready-to-use chromogenic medium on 90mm plates for the isolation and direct differentiation of Staphylococcus aureus
- Code: 01018
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Staph aureus Agar is a selective chromogenic medium designed for the direct qualitative detection, differentiation, and presumptive identification of Staphylococcus aureus from swabs of teguments, wounds, or soft tissue specimens.
- The medium may also serve as an early warning indicator in diagnostic testing for infections, highlighting the possible presence of S. aureus. Concomitant cultures are recommended for recovering organisms for further microbiological testing or epidemiological typing. Additionally, BacterChrom™ Staph aureus Agar is applicable for detecting S. aureus in food products, animal feed, and environmental samples.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterChrom™ Staph aureus Agar employs chromogenic substrates that enable differentiation of target microorganisms based on colony color. Selective agents in the medium suppress non-target organisms, ensuring specificity. The chromogenic system highlights enzymatic activity unique to S. aureus, allowing for rapid and clear visual identification.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate at 35 – 37 °C for 18 – 24 hours in aerobic conditions.
RESULTS
Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| S. aureus | pink to mauve |
| Other bacteria | inhibited, colourless, blue |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. aureus ATCC 25923 | Incubate for 18 – 24 hours at 35 – 37 °C | Mauve colonies |
| S. saprophyticus ATCC 15305 | Blue colonies | |
| E. coli ATCC 35218 | Inhibited | |
| E. faecalis ATCC 29212 | Inhibited |
For 1 liter of medium
| Agar | 15,0 g |
| Peptones and yeast extract | 40,0 g |
| Salt | 25,0 g |
| Chromogenic mix | 2,5 g |
pH of the ready-to-use medium at 25°C: 6,9 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Staph aureus Agar | 01018 | 10 plates/box or as per customer request. |
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