BacterChrom™ STEC Agar Plates

Ready-to-use chromogenic medium on 90mm plates for qualitative analysis of Shiga toxin-producing Escherichia coli (STEC).
Code: 01014
Packaging: 10 plates/box or as per customer requirements.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 03 months from the date of manufacture.

INTENDED USE

BacterChrom™ STEC Agar is a selective chromogenic culture medium intended for use in the qualitative direct detection, differentiation and presumptive identification of Shiga-like-toxin producing Escherichia coli (STEC), to aid in the diagnosis of STEC infections.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterChrom™ STEC Agar differentiates microorganisms based on specific enzymatic activities that trigger distinct chromogenic reactions. These reactions result in visually distinguishable colony colors, enabling rapid presumptive identification of STEC while inhibiting non-target organisms. The selective components ensure high specificity by minimizing interference from competing flora.

Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
Direct streaking sample onto the plate
Incubate in aerobic conditions at 35 – 37°C for 18 – 24 hours

RESULTS

Microorganism	Typical colony apprearance
STEC	Mauve
Other Enterobacteriaceae	colourless, blue or inhibited
Gram (+) bacteria	inhibited
*Note: fluorescence under UV lamp (365nm.) :*
STEC O157	non fluorescent
STEC non O157	+/- fluorescent

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.