BacterChrom™ Strep A Agar
- Ready-to-use chromogenic medium on 90mm plates for qualitative analysis and differentiation of Streptococcus pyogenes – Group A Streptococcus (GAS) in throat infection samples.
- Code: 01017
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterChrom™ Strep A Agar is a selective chromogenic culture medium designed for the qualitative detection, differentiation, and presumptive identification of Group A Streptococcus (Streptococcus pyogenes). It aids in diagnosing throat infections caused by this pathogen using throat swabs. Results are observed after 18–24 hours of incubation at 35–37°C in a CO₂ atmosphere.
- This medium is not intended for diagnosing infections, nor for monitoring or guiding treatment. Concomitant cultures are necessary for further microbiological testing or epidemiological typing.
PRINCIPLES
- BacterChrom™ Strep A Agar uses specific chromogenic substrates to detect enzymatic activity unique to target microorganisms, producing distinct colony colors. The selective agents in the medium inhibit non-target organisms, ensuring high specificity and clear visual differentiation of Group A Streptococcus. This enables rapid presumptive identification and supports efficient laboratory workflows.
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Direct streaking sample onto the plate
- Incubate at 35 – 37 °C for 18 – 24 hours in a CO2 atmosphere
RESULTS
- Qualitative reading and interpretation of the plates:
| Microorganism | Typical colony apprearance |
| Streptococcus pyogenes (group A) | orange to red |
| Other Streptococcus | steel blue or colourless |
| Other Gram (+) bacteria | inhibited |
| Yeasts | inhibited |
| Gram (-) bacteria | inhibited |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| S. pyogenes ATCC 19615 | Incubate for 18 – 24 hours at 35 – 37 °C, 5% CO2 | Orange to red colonies |
| E. faecalis ATCC 29212 | Metallic blue colonies | |
| E. coli ATCC 35218 | Inhibited | |
| S. aureus ATCC 25923 | Inhibited |
For 1 liter of medium
| Agar | 15,0 g |
| Peptones and yeast extract | 27,0 g |
| Salt | 9,0 g |
| Chromogenic and selective mix | 0,3 g |
| Growth factors | 3,0 g |
| Supplement (S1) | 2mL |
| Supplement (S2) | 0,1 g |
pH of the ready-to-use medium at 25°C: 7,2 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterChrom™ Strep A Agar | 01017 | 10 plates/box or as per customer request. |
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