BacterContact™ MacConkey Agar

Ready-to-use medium on 60mm plates for surface microbiological sampling to detect Enterobacteriaceae
Code: 12013
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 06 months from the date of manufacture.

INTENDED USE

BacterContact™ MacConkey Agar for surface microbiological sampling to detect Enterobacteriaceae.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

BacterContact™ MacConkey Agar: Bile salts and crystal violet inhibit the growth of gram-positive bacteria. The dye primarily inhibits the growth of enterococci and staphylococci. Lactose fermentation into acid is indicated by neutral red, resulting in red or pink colonies. Lactose-negative colonies form colorless colonies.

Prepare the surface to be tested: Clean the surface to be tested using a 70% alcohol solution or another cleaning solution. Then, wait for the surface to dry completely.
Open the pre-packaged BacterContact plates: Ensure that the packaging of the plates is not torn or damaged before opening.
Place the Contact plate on the surface to be tested: Press the Rodac plate onto the surface to be tested. The recommended contact time between the plate and the test surface is 10 seconds with a pressing force of 500g.
Seal the Rodac plate: Make sure that the lid of the Rodac plate is tightly closed. Wipe the surface again with 70% alcohol.
- 30 – 35 °C for 72 ± 18–24 hours.

- 30 – 35 °C for 72 ± 6 hours (NF EN ISO 21149, NF EN ISO 18415)
- 20 – 25 °C for 3 to 5 days for Total Microbial Count (Pharmacopoeia)

RESULTS

After incubation for the required period, typically 18–24 hours, the plates are examined for the presence of microorganisms. The results are assessed by counting the number of microbial colonies on the plates.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Microorganisms	Incubation conditions	Expected results
E. coli ATCC 35218	18 – 24 hours of incubation at 35 – 37 °C	P_R ≥ 70 %

For 1 liter of medium

Pancreatic digest of gelatin	17,0 g
Tryptone	1,5 g
Peptic digest of meat	1,5 g
Lactose	10,0 g
Bile salts	1,5 g
Sodium chloride	5,0 g
Neutral red	30,0 mg
Crystal violet	1,0 mg
Agar	13,5 g