INTENDED USE
- BacterContact™ Plate Count Agar for surface microbiological sampling.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterContact™ Plate Count Agar is the nutrients are provided by tryptone, vitamins from yeast extract, and glucose, which is used as an energy source to support the growth of most bacteria.
- Prepare the surface to be tested: Clean the surface to be tested using a 70% alcohol solution or another cleaning solution. Then, wait for the surface to dry completely.
- Open the pre-packaged BacterContact plates: Ensure that the packaging of the plates is not torn or damaged before opening.
- Place the Contact plate on the surface to be tested: Press the Rodac plate onto the surface to be tested. The recommended contact time between the plate and the test surface is 10 seconds with a pressing force of 500g.
- Seal the Rodac plate: Make sure that the lid of the Rodac plate is tightly closed. Wipe the surface again with 70% alcohol.
- Evaluation of Results: Incubate under the following conditions
- 30 – 35 °C for 72 ± 6 hours (NF EN ISO 21149, NF EN ISO 18415)
- 20 – 25 °C for 3 to 5 days for Total Microbial Count (Pharmacopoeia)
RESULTS
- After incubation for the required period, usually from 3 to 5 days, the plates are examined for the presence of fungi. The results are evaluated by counting the number of fungal colonies on the plates
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
Microorganisms | Incubation conditions | Expected results |
S. aureus ATCC 25923 | 20 – 24 hours of incubation at 30 – 35 °C | PR ≥ 70 % |
E. coli ATCC 35218 | PR ≥ 70 % | |
C. albicans ATCC 10231 | PR ≥ 50 % | |
A. brasilliensis ATCC 16404 | 72 hours of incubation at 20 – 25 °C | PR ≥ 50 % |
For 1 liter of medium
Trypton | 5,0 g |
Yeast extract | 2,5 g |
Glucose | 1,0 g |
Agar | 15,0 g |
pH of the ready-to-use media at 25 °C: 7 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
Product Name | Code | Packaging |
BacterContact™ Plate Count Agar | 12010 | 10 plates/ box or as per customer request. |
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