12001-BacterContact™ SDA có Chloramphenicol

BacterContact™ Sabouraud CAF Agar + Penase + Cephalosporinase + Neutralizing

Ready-to-use medium on 60mm plates for enumeration of yeasts and moulds on sanitized surface and personnelwith inactivation of β-lactam antibiotics and disinfectants.
Code: 4109024
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 06 months from the date of manufacture.

INTENDED USE

BacterContact™ Sabouraud CAF Agar + Penase + Cephalosporinase + Neutralizing for enumeration of yeasts and moulds on sanitized surface and personnelwith inactivation of β-lactam antibiotics and disinfectants.

The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

Pancreatic digest of casein and peptic digest of animal tissue provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients which support the growth of microorganism. The high concentration of dextrose along with the low pH of the medium promotes the growth of fungi while inhibiting most of bacteria. Agar is the solidifying agent. Histidine inactivates aldehydes. Lecithin neutralizes quaternary ammonium compounds. Polysorbate 80 (Tween 80) is effective against phenolic compounds and mercurial derivates. Sodium thiosulfate neutralizes halogen compounds. Penase is a preparation of Pencillinase and with

Cephalosporinase inactivate β-lactam antibiotics (penicillins and cephalosporins).

1 Levy Unit (LU) is defined as the amount of penicillinase that inactivates 59.3 IU of Penicillin G per hour at pH 7.0 at 25°C.
1 International Unit (IU) is defined as the amount of enzyme needed to hydrolyze 1 µmole of Penicillin G (Penicillinase) or 1 µmole of Cephalosporin C (Cephalosporinase) per minutes at pH 7.0 at 25°C.

Preparation:

Prepare a test diagram for the areas that are to be tested and label plates with the corresponding location identification. Ensure that the label cannot be readily wiped off or removed. RODAC plates are prepared so that the agar surface is convex for sampling flat surfaces. Prior to sampling, the plates should be warmed to room temperature in the plastic sleeve for approximately 15 – 20 minutes with agar up and the lid down. Remove the quantity of plates from the sleeve that are required for testing. The location/ site identification should be written on the base (agar portion, not the lid) of the plate.

Sampling:

While wearing gloves, remove the lid from the plate with one hand. With the other hand, hold the base (agar portion of the plate) with thumb and middle finger. Use the index finger to gently press the plate on the test surface. Make sure the entire agar surface touches the test surface. Do not move the plate laterally while sampling as it will spread contaminants making enumeration difficult. Place the lid back on the plate and tape closed. Be sure to clean the test area after sampling to remove any residual growth media remaining on the surface.
For total yeast and mold count (Microbial Limit Test), plates should be incubated for 5 to 7 days at 20 – 25°C (EP/USP) prior to colony counting.

RESULTS

Observe daily for the formation of colonies.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Candida albicans ATCC 10231	Incubate for 72h at 20 – 25^oC	Good growth
Aspergillus brasiliensis ATCC 16404	Incubate for 72h at 20 – 25^oC	Good growth

For 1 liter of medium (refrance)

Dextrose	40,0 g
Pancreatic digest of animal tissues	5,0 g
Pancreatic digest of casein	5,0 g
Chloramphenicol	50,0 mg
Agar	15,0 g
Histidine	1,0 g
Lecithin	0,7 g
Polysorbate 80	5,0 g
Sodium Thiosulfate	0,5 g
Penase	1,0 mL
Cephalosporinase	1,0 mL