12001-BacterContact™ SDA có Chloramphenicol

BacterContact™ Sabouraud Dextrose Agar + 0.1% Penase + Neutralizing

Ready-to-use medium on 60mm plates for detection of yeasts and moulds with inactivation of disinfectants.
Code: 4109025
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 06 months from the date of manufacture.

INTENDED USE

BacterContact™ Sabouraud Dextrose Agar + 0.1% Penase + Neutralizing for the determination of total aerobic viable count of yeasts and moulds in procedures for environmental and personnel hygiene monitoring.

The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

Pancreatic digest of casein and peptic digest of animal tissue provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients which support the growth of microorganism. Dextrose is an energy source. Agar is the solidifying agent. The high concentration of dextrose and the acidic pH of the medium permit selectivity of fungi. Histidine inactivates aldehydes. Lecithin neutralizes quaternary ammonium compounds. Polysorbate 80 (Tween 80) is effective against phenolic compounds and mercurial derivates. Sodium thiosulfate neutralizes halogen compounds. Penase is a preparation of penicillinase for inactivating residuals of penicillins.

1 Levy Unit (LU) is defined as the amount of penicillinase that inactivates 59.3 IU of Penicillin G per hour at pH 7.0 at 25°C.
1 International Unit (IU) is defined as the amount of enzyme needed to hydrolyze 1 µmole of Penicillin G (Penicillinase) per minute at pH 7.0 at 25°C.

Preparation:

Prepare a test diagram for the areas that are to be tested and label plates with the corresponding location identification. Ensure that the label cannot be readily wiped off or removed. RODAC plates are prepared so that the agar surface is convex for sampling flat surfaces. Prior to sampling, the plates should be warmed to room temperature in the plastic sleeve for approximately 15 – 20 minutes with agar up and the lid down. Remove the quantity of plates from the sleeve that are required for testing. The location/ site identification should be written on the base (agar portion, not the lid) of the plate.

Sampling:

While wearing gloves, remove the lid from the plate with one hand. With the other hand, hold the base (agar portion of the plate) with thumb and middle finger. Use the index finger to gently press the plate on the test surface. Make sure the entire agar surface touches the test surface. Do not move the plate laterally while sampling as it will spread contaminants making enumeration difficult. Place the lid back on the plate and tape closed. Be sure to clean the test area after sampling to remove any residual growth media remaining on the surface.
For total yeast and mold count (Microbial Limit Test), plates should be incubated for 5 to 7 days at 20 – 25°C (EP/USP) prior to colony counting.

RLTSESU

Observe daily for the formation of colonies.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Candida albicans ATCC 10231	Incubate for 72h at 20 – 25^oC	Good growth (P_R ≥ 0,7)
Aspergillus brasiliensis ATCC 16404
Staphylococcus aureus ATCC 25923	Incubate for 24h at 30 – 35^oC
Escherichia coli ATCC 25922

For 1 liter of medium (refrance)

Dextrose	40,0 g
Pancreatic digest of animal tissues	5,0 g
Pancreatic digest of casein	5,0 g
Agar	15,0 g
Histidine	1,0 g
Lecithin	0,7 g
Polysorbate 80	5,0 g
Sodium Thiosulfate	0,5 g
Penase	1,0 mL

pH of the ready-to-use medium at 25°C: 5,6 ± 0,2

Product Name	Code	Packaging
BacterContact™ Sabouraud Dextrose Agar + 0.1% Penase + Neutralizing	4109025	10 plates/ box or as per customer request.