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BacterContact™ Sabouraud Dextrose Agar + LTHTh
- Ready-to-use medium on 60mm plates for the determination of the total count of yeasts and molds
- Code: 12005
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
INTENDED USE
- BacterContact™ Sabouraud Dextrose Agar + LTHTh for isolating microorganisms from surfaces disinfected with quaternary ammonium compounds.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- BacterContact™ Sabouraud Dextrose Agar + LTHTh with a pH of approximately 5,6 which promotes the growth of fungal species, particularly dermatophytes, while inhibiting the growth and development of other bacteria in the sample. The product contains peptone, which provides nutrients such as amino acids and nitrogen for growth and development. Dextrose serves as a carbon source and is considered an energy source. Lecithin (L), Tween 80 (T), histidine (H), and sodium thiosulfate (Th) act as neutralizers for residual disinfectant chemicals.
- Prepare the surface to be tested: Clean the surface to be tested using a 70% alcohol solution or another cleaning solution. Then, wait for the surface to dry completely.
- Open the pre-packaged BacterContact plates: Ensure that the packaging of the plates is not torn or damaged before opening.
- Place the Contact plate on the surface to be tested: Press the Rodac plate onto the surface to be tested. The recommended contact time between the plate and the test surface is 10 seconds with a pressing force of 500g.
- Seal the Rodac plate: Make sure that the lid of the Rodac plate is tightly closed. Wipe the surface again with 70% alcohol.
- Evaluation of Results: Incubate under the following conditions
- 30 – 35 °C for 72 ± 6 hours (NF EN ISO 21149, NF EN ISO 18415)
- 20 – 25 °C for 3 to 5 days for Total Microbial Count (Pharmacopoeia)
RESULTS
- After incubation for the required period, usually from 3 to 5 days, the plates are examined for the presence of fungi. The results are evaluated by counting the number of fungal colonies on the plates
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Microorganisms | Incubation conditions | Expected results |
| C. albicans ATCC 10231 | 24 – 48 hours of incubation at 30 – 35°C | PR ≥ 50 % |
| S. cerevisiae ATCC 20827 | ||
| A. brasiliensis ATCC 16404 | 72 hours of incubation at 20 – 25°C | PR ≥ 70 % |
For 1 liter of medium
| Dextrose | 40,0 g |
| Pancreatic digest of animal tissues | 5,0 g |
| Pancreatic digest of casein | 5,0 g |
| Polysorbate (Tween) 80 | 5,0 ml |
| Lecithin | 0,7 g |
| Histidine | 0,5 g |
| Natri thiosulfate | 0,05 g |
| Agar | 15,0 g |
pH of the ready-to-use media at 25 °C: 5,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterContact™ Sabouraud Dextrose Agar + LTHTh | 12005 | 10 plates/ box or as per customer request. |
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