12001-BacterContact™ SDA có Chloramphenicol

BacterContact™ Sabouraud Dextrose Agar + Neutralizing

BacterContact™ Sabouraud Dextrose Agar + Neutralizing
Code: 4109027
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 06 months from the date of manufacture.

INTENDED USE

BacterContact™ Sabouraud Dextrose Agar + Neutralizing is a non selective isolation medium used for the determination of total aerobic viable count of yeasts and moulds in surfaces testing and air microbial control by dynamic sampling technique. The composition of the base culture medium complies with the recommendations of the current United States, European and Japanese Pharmacopeia. In addition, neutralizing agents are included in the medium to inactivate residual disinfectants allowing detection of microorganisms surviving after treatment of surface and material with antiseptics.

The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

Pancreatic digest of casein and peptic digest of animal tissue provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients which support the growth of microorganism. Dextrose is an energy source. Agar is the solidifying agent. The high concentration of dextrose and the acidic pH of the medium permit selectivity of fungi. Histidine inactivates aldehydes. Lecithin neutralizes quaternary ammonium compounds. Polysorbate 80 (Tween 80) is effective against phenolic compounds and mercurial derivates. Sodium thiosulfate neutralizes halogen compounds.

Preparation:

Prepare a test diagram for the areas that are to be tested and label plates with the corresponding location identification. Ensure that the label cannot be readily wiped off or removed. RODAC plates are prepared so that the agar surface is convex for sampling flat surfaces. Prior to sampling, the plates should be warmed to room temperature in the plastic sleeve for approximately 15 – 20 minutes with agar up and the lid down. Remove the quantity of plates from the sleeve that are required for testing. The location/ site identification should be written on the base (agar portion, not the lid) of the plate.

Sampling:

While wearing gloves, remove the lid from the plate with one hand. With the other hand, hold the base (agar portion of the plate) with thumb and middle finger. Use the index finger to gently press the plate on the test surface. Make sure the entire agar surface touches the test surface. Do not move the plate laterally while sampling as it will spread contaminants making enumeration difficult. Place the lid back on the plate and tape closed. Be sure to clean the test area after sampling to remove any residual growth media remaining on the surface.
For total yeast and mold count (Microbial Limit Test), plates should be incubated for 5 to 7 days at 20 – 25°C (EP/USP) prior to colony counting.

RESULTS

Observe for the formation of fungal colonies exhibiting typical microscopic and colonial morphology. Record the number of CFU per plate. Colonies should be further isolated and identified with appropriate procedures.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
Candida albicans ATCC 10231	Incubate for 72h at 20 – 25^oC	Good growth, 50-200% recovery
Aspergillus brasiliensis ATCC 16404
Saccharomyces cerevisiae ATCC 9763

For 1 liter of medium (refrance)

Dextrose	40,0 g
Pancreatic digest of animal tissues	5,0 g
Pancreatic digest of casein	5,0 g
Agar	15,0 g
Histidine	1,0 g
Lecithin	0,7 g
Polysorbate 80	5,0 g
Sodium Thiosulfate	0,5 g