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BacterContact™ SDA with Chloramphenicol
- Ready-to-use medium on 60mm plates for the determination of the total count of yeasts and molds.
- Code: 12001
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
INTENDED USE
- BacterContact™ SDA with Chloramphenicol is recommended for the isolation and the enumeration of yeasts and molds, especially when the samples are highly contaminated with bacteria. It is also used as selective isolation media for Candida albicans in cosmetic products.
- The typical composition corresponds to that defined in the standards NF EN ISO 18416 and NF EN ISO 16212.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Peptic digest of Meat is the nitrogen source for growth. Glucose is an energy source. Chloramphenicol is a heat-stable, broad spectrum antibiotic which inhibits the development of contaminating microflora
- Prepare the surface to be tested: Clean the surface to be tested using a 70% alcohol solution or another cleaning solution. Then, wait for the surface to dry completely.
- Open the pre-packaged BacterContact plates: Ensure that the packaging of the plates is not torn or damaged before opening.
- Place the Contact plate on the surface to be tested: Press the Rodac plate onto the surface to be tested. The recommended contact time between the plate and the test surface is 10 seconds with a pressing force of 500g.
- Seal the Rodac plate: Make sure that the lid of the Rodac plate is tightly closed. Wipe the surface again with 70% alcohol.
- Evaluation of Results: Incubate under the following conditions
- 30 – 35 °C for 72 ± 6 hours (NF EN ISO 21149, NF EN ISO 18415)
- 20 – 25 °C for 3 to 5 days for Total Microbial Count (Pharmacopoeia)
RESULTS
- After incubation for the required period, usually from 3 to 5 days, the plates are examined for the presence of fungi. The results are evaluated by counting the number of fungal colonies on the plates
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Microorganisms | Incubation conditions | Expected results |
| C. albicans ATCC 10231 | 3 – 5 days of incubation at 30 °C | PR ≥ 70 % |
| S. cerevisiae ATCC 20827 | ||
| A. brasiliensis ATCC 16404 | ||
| E. coli ATCC 35218 | Inhibited |
For 1 liter of medium
| Dextrose | 40,0 g |
| Pancreatic digest of animal tissues | 5,0 g |
| Pancreatic digest of casein | 5,0 g |
| Chloramphenicol | 50,0 mg |
| Agar | 15,0 g |
pH of the read-to-use media at 25 °C: 5,6 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterContact™ SDA with Chloramphenicol | 12001 | 10 plates/ box or as per customer request. |
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