BacterPlate™ Columbia Agar (CBA)
- Ready-to-use chromogenic medium on 90mm plates for culturing and isolating of fastidious microorganisms
- Code: 05008
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
INTENDED USE
- BacterPlate™ Columbia Agar is a highly nutritive medium used for the growth and isolation of a large variety of microorganisms, particularly very fastidious bacteria: streptococci and pneumococci in animal samples. When blood, selective agents or growth accelerators are added, it becomes possible to prepare a wide variety of media adapted to specific uses.
- The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.
PRINCIPLES
- Peptones included in the composition of the medium favor the excellent growth of colonies.
- Yeast extract is a source of vitamin B complex.
- Starch is a detoxifying agent and also an energy source.
- Defibrinated sheep blood, which can be added to the medium, favors the detection of hemolytic reactions and supplies X factor (heme) required for the growth of a large number of bacteria, but lacks V factor (nicotinamide adenine dinucleotide) due to the presence of an NADase, which destroys any NAD present. Haemophilus influenzae, which requires both X and V factors, does not grow on agar containing ordinary blood.
- The following media can be prepared with Columbia base :
- Blood agar: By adding 5 or 10% sterile sheep blood after autoclaving and cooling, the medium is suitable for the growth of Streptococcus, Pneumococcus, Staphylococcus, Listeria and Erysipelothrix. It can be made selective by adding colistin and nalidixic acid to preclude the development of Gram-negative bacteria and Bacillus.
- Chocolate agar: By adding 10% sheep or horse blood to sterile Colombia Agar and heating to 70°C for 5 minutes until a chocolate color develops, an excellent medium is obtained for growth of Haemophilus, Neisseria, Taylorella or Campylobacter.
- Base media without enrichment: Columbia Agar can be used to grow Brucella abortus, Yersinia pestis and Clostridium perfringens, as well as all enterobacteria
- Allow to warm to room temperature before inoculation, dry the plates in an incubator with the covers partially removed.
- Inoculate in order to obtain isolated colonies.
- Incubate at 37°C for 24 to 48 hours in optimal conditions for the culture of the inoculated germs
RESULTS
Observe the bacterial growth.
- Beta hemolysis
- Streptococci belonging to Lancefield group A appear as small, grey colonies, translucid or opaque, surrounded by a zone of beta hemolysis. Other bacteria may present the same type of hemolysis: Listeria, hemolytic Staphylococci, Escherichia coli and Pseudomonas.
- Staphylococci appear as opaque, yellow-gold or white colonies, with or without type β hemolysis zones.
- Listeria present small zones of beta hemolysis.
- Bacillus cereus form a clear zone surrounding the colonies.
- Alpha hemolysis
- Pneumococci appear as flat, shiny, grey and occasionally mucoid colonies surrounded by a zone of narrow, greenish hemolysis referred to as alpha hemolysis.
- CAMP Factor
- Group B Streptococci produce an extracellular, thermoresistant substance (CAMP Factor) which provokes a triangle of total hemolysis in a zone of incomplete staphylococcal hemolysis, at the junction of the two cultures.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
Reference strains | Incubation conditions | Expected results |
E. coli ATCC 35218 | Incubate for 24 – 48 hours at 37°C | Good growth |
S. aureus ATCC 25923 | Good growth |
For 1 liter of medium
Special peptone | 23,0 g |
Starch | 1,0 g |
Sodium chloride | 5,0 g |
Bacteriological agar | 13,5 g |
Gentamycine | 0,02 g |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
Product Name | Code | Packaging |
BacterPlate™ Columbia Agar | 05008 | 10 plates/ box or as per customer request. |
Related products
BacterContact™ Tryptic Soy Agar + 0.1% Penase + Neutralizing
- Ready-to-use medium on 60mm plates for environmental and personnel monitoring with inactivation of penicillins and disinfectants.
- Code: 4109028
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
SwabCollect™ D/E Neutralizing Broth, 1 mL
- Microbial Sampling Tool for surface sampling, using a swab and a plastic tube containing D/E Neutralizing Broth to recover and protect microorganisms, during transport and analysis.
- Code: 4108029
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.
SwabCollect™ Neutralizing Buffer, 4 mL
- Microbial Sampling Tool for surface sampling, using a swab and a plastic tube containing BPW (Buffered Peptone Water) to recover and protect microorganisms during transport and analysis.
- Code: 4108006
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.
SwabCollect™ Cary Blair without Charcoal, 5mL
- Recommended for collection and shipment of clinical specimens.
- Code: 4106010
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.
SwabCollect™ Neutralizing Buffer, 1 mL
- Microbial Sampling Tool for surface sampling, using a swab and a plastic tube containing Neutralizing Buffer to neutralize the effects of residual disinfectants and protect microorganisms during transport and analysis.
- Code: 4108005
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.
SwabCollect™ Stuart Amies without Charcoal, 5mL
- Used for transportation and preservation of microbiological specimen.
- Code: 4106008
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.
BacterChrom™ O157 Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for the isolation and differentiation of E. coli O157 in food and clinical samples.
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
Categories: Biopharma, Cosmetics, Culture media, Ready-to-use 90mm agar plates
BacterContact™ Sabouraud Dextrose Agar + Lactamator 500 IU + Neutralizing
- Ready-to-use medium on 60mm plates for enumeration of yeasts and moulds on sanitized surface and personnel.
- Code: 4109026
- Packaging: 10 plates/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 06 months from the date of manufacture.
BacterChrom™ B. cereus Agar Plates
- Ready-to-use chromogenic medium on 90mm plates for detection and enumeration of Bacillus cereus group.
- Code: 01031
- Packaging: 10 plates/box or as per customer requirements.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 03 months from the date of manufacture.
SwabCollect™ D/E Neutralizing Broth, 10 mL
- Microbial Sampling Tool for surface sampling, using a swab and a plastic tube containing D/E Neutralizing Broth to recover and protect microorganisms, during transport and analysis.
- Code: 4108032
- Packaging: 50 set/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 18 months from the date of manufacture.