BacterPlate™ ECC Agar

Ready-to-use medium on 90mm plates for the detection and enumeration of Escherichia coli and coliforms
Code: 05002
Packaging: 10 plates/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 04 months from the date of manufacture.

INTENDED USE

BacterPlate™ ECC Agar s a selective agar for the simultaneous and specific enumeration without confirmation of Escherichia coli and of other coliform bacteria in human and animal food.
The packaging with semi-permeable Cellophane film helps balance the humidity of the environment during storage.

PRINCIPLES

The classification of coliforms is traditionally founded on their capacity to ferment lactose with a corresponding production of acid. The fermentation of lactose results from the successive cascade effect of two enzymes : first a permease responsible for the penetration of the sugar into the bacteria, and then a β-galactosidase which cuts the glucose to galactose, thereby actively entering into the fermentation process.
In 1989, Leclerc & Mossel proposed that the presence of β – galactosidase with coliforms be used as the main criteria for classification. The use of a synthetic chromogenic substrate, insensitive to variations in the permeability of lactose, allows the use of this enzyme by a colorimetric reaction.
94 to 97% of Escherichia coli possess a β-D-glucuronidase activity and that the same activity is only rarely encountered with other species (enzyme activity has been detected in a small number of strains of Citrobacter, Enterobacter, Klebsiella, Salmonella, Shigella and in Yersinia)

Allow the agar plates to stabilize at room temperature. Dry the plates in an incubator by partially opening the lids.
Streak the sample onto the agar plates using a sterile inoculating loop.
Incubate the plates at 35 ± 2°C for 18 – 24 hours

RESULTS

After incubation, count the number of colonies on plates containing fewer than 300 colonies.
Coliforms other than Escherichia coli produce pink colonies.
Colonies of Escherichia coli appear blue to violet and may sometimes exhibit a diffuse pink halo around the colonies.

BacterLab ensures the quality of each product batch by testing with WDCM reference strains.

For 1 liter of medium

pH of the ready-to-use medium at 25°C: 6,9 ± 0,2

*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.

Product Name	Code	Packaging
BacterPlate™ ECC Agar	05002	10 plates/ box or as per customer request.