BacterSerum™ Freeze-dried rabbit plasma

MỤC ĐÍCH SỬ DỤNG

BacterSerum™ Freeze-dried rabbit plasma use the coagulase test to differentiate Staphylococcus aureus from Coagulase negative Staphylococcus.

NGUYÊN TẮC

Staphylococcus aureus produces two types of coagulase:
- Extracellular coagulase reacts with prothrombin;
- Bound coagulase, which adheres to the bacterial wall, reacts with fibrinogen to produce coagulation.
In tests, free coagulase reacts primarily by forming clots in the plasma, indicating a positive reaction. In cases of coagulase-negative staphylococci, screening for the presence of other enzymes such as phosphatase or deoxyribonuclease, which are also indicators of pathogenicity, should be performed

Inoculate the BacterTube™ BHI Broth for confirmation and incubate for 24 hours at 37 °C.
Prepare a turbidity of 0.5McF of the sample to be tested.
Inoculate 0.1 mL of the BacterTube™ BHI Broth into a test tube containing rabbit plasma.
Mix well.
Incubate at 35°C or 37 °C for 4 – 24 hours.

Note:

As a control, add 0.1 mL of sterile BacterTube™ BHI Broth to 0.3 mL of BacterSerum™ Freeze-dried rabbit plasma. This negative control showed no signs of clotting after 24 hours of incubation.

RESULTS

BacterLab performs quality control on each product batch.

PERFORMANCE	STANDARD
Culture conditions 35 – 37^oC after 4 – 6 hours, with inoculum 10³ – 10⁴ CFU/ mL
S.aureus ATCC 25923	Positive
S.epidermidis ATCC 12228	Negative