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BacterTube™ BHI có 20% Glycerol
- The medium contained in a screw-cap tube with 1 mL used for preserving bacteria and fungi.
- Code: 08012
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ BHI 20% Glycerol is used for the preservation of organisms by freezing. It is recommended for long-term storage and ensures the viability of microorganisms under low-temperature conditions
PRINCIPLES
- The Brain Heart Infusion (BHI) medium provides essential nutrients required for the survival of microorganisms. A 20% concentration of glycerol is added to the broths to maintain viability of microorganisms and to protect cell membranes. Glycerol acts as a cryoprotective agent and allows for long-term bacterial preservation
- Open the screw-cap tube containing the BHI 20% glycerol in in a sterile environment.
- Use a sterile swab to collect bacteria or fungi from the surface of an agar plate or add the microbial suspension to the tube (not exceeding the total capacity)
- Mix gently to ensure even distribution
- Freeze the tubes at temperatures appropriate for your laboratory storage (-20°C or -80°C is recommended).
Note: Ensure the tube is tightly closed to prevent contamination or evaporation during storage
RESULTS
- Growth of frozen stock culture on solid media indicates the presence of a viable organism
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Escherichia coli ATCC 25922 | 18 – 24 hours | Good growth |
| Staphylococcus aureus ATCC 25923 | Good growth |
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Escherichia coli ATCC 25922 | 18 – 24 hours | Good growth |
| Staphylococcus aureus ATCC 25923 | Good growth |
| Product Name | Code | Packaging |
| BacterTube™ BHI 20% Glycerol | 08012 | 50 tubes/ box or as per customer request. |
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