BacterTube™ BHI with XV

A nutrient-rich medium that provides essential substances for microbial growth, suitable for culturing various types of microorganisms.
Code: 08010
Packaging: 50 tubes/ box or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 6 months from the date of manufacture.

INTENDED USE

BacterTube™ BHI with XV is a non-selective enrichment medium that supports the growth of all bacteria, including Haemophilus influenzae. It is designed to promote the growth of a wide range of microorganisms, without inhibiting any specific bacterial species.

PRINCIPLES

BacterTube™ BHI with XV is formulated to provide essential nutrients to support the growth of a broad range of microorganisms, particularly those with complex nutritional requirements. The composition includes:
- Brain-Heart Infusion: Provides rich nutritive compounds to support fastidious bacterial growth.
- IsoVitaleX™ Enrichment: A supplement containing vitamins, amino acids, and other growth factors to enhance the growth of microorganisms, especially for those with complex nutrient needs.
- Sodium chloride: Maintains osmotic balance in the medium.
- Disodium phosphate: Acts as a buffering agent to maintain pH stability.
- Glucose: Serves as an energy source to promote the growth of microorganisms.

Directly inoculate the specimen or sample into the medium.
Incubate at 35 – 37°C for 4 – 8 hours.
Perform subculturing on specialized agar plates containing selective media and incubate under appropriate conditions

RESULTS

Positive Test: A positive result is indicated by the formation of a mass within 24 hours.
Negative Test: A negative result is indicated when no mass is formed after 24 hours.

BacterLab ensures the quality of each product batch by testing with ATCC reference strains.

Reference strains	Incubation conditions	Expected results
H. influenzae ATCC 49766	12 – 24 hours, 35 – 37°C, 5% CO₂	Growth and form a mass

For 1 liter of medium