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BacterTube™ BHI with XV
- A nutrient-rich medium that provides essential substances for microbial growth, suitable for culturing various types of microorganisms.
- Code: 08010
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 6 months from the date of manufacture.
INTENDED USE
- BacterTube™ BHI with XV is a non-selective enrichment medium that supports the growth of all bacteria, including Haemophilus influenzae. It is designed to promote the growth of a wide range of microorganisms, without inhibiting any specific bacterial species.
PRINCIPLES
- BacterTube™ BHI with XV is formulated to provide essential nutrients to support the growth of a broad range of microorganisms, particularly those with complex nutritional requirements. The composition includes:
- Brain-Heart Infusion: Provides rich nutritive compounds to support fastidious bacterial growth.
- IsoVitaleX™ Enrichment: A supplement containing vitamins, amino acids, and other growth factors to enhance the growth of microorganisms, especially for those with complex nutrient needs.
- Sodium chloride: Maintains osmotic balance in the medium.
- Disodium phosphate: Acts as a buffering agent to maintain pH stability.
- Glucose: Serves as an energy source to promote the growth of microorganisms.
- Directly inoculate the specimen or sample into the medium.
- Incubate at 35 – 37°C for 4 – 8 hours.
- Perform subculturing on specialized agar plates containing selective media and incubate under appropriate conditions
RESULTS
- Positive Test: A positive result is indicated by the formation of a mass within 24 hours.
- Negative Test: A negative result is indicated when no mass is formed after 24 hours.
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| H. influenzae ATCC 49766 | 12 – 24 hours, 35 – 37°C, 5% CO2 | Growth and form a mass |
For 1 liter of medium
| Brain infusion solids | 12,5 g |
| Beef heart infusion solids | 5,0 g |
| Proteose peptone | 10,0 g |
| Sodium chloride | 5,0 g |
| Disodium phosphate | 2,5 g |
| IsoVitaleX™ Enrichment | 1 vial |
pH of the ready-to-use medium at 25°C: 7,4 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ BHI with XV | 08010 | 50 tubes/ box or as per customer request. |
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