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BacterTube™ Mueller Hinton Broth (MHB)
- Ready-to-use liquid medium on 90mm plates for non-selective cultivation a large number of microorganisms of varied origins.
- Code: 08026
- Packaging: 50 tubes/ box or as per customer request.
- Storage Conditions: 2–8°C.
- Transportation: Ambient temperature.
- Shelf Life: 3 months from the date of manufacture.
INTENDED USE
- BacterTube™ Mueller Hinton Broth (MHB) is a non-selective medium for the culture of a large number of microorganisms of varied origins, as well as for determining minimal inhibitory concentrations by the dilution method
PRINCIPLES
- The choice of ingredients is determined in order to obtain a very small quantity of thymine and thymidine, substances known to inhibit antibacterial activity of trimethoprim, a very small quantity of para-aminobenzoic acid (PABA) and its structural analogues, which antagonize the activity of sulfonamides. Beef infusion and acid hydrolysate of casein are sources of amino acids and other nitrogenous substances favoring the growth of microorganisms. Starch acts as a detoxifying agent.
- Cultures in tubes :
- Inoculate the medium with purified cultures or with other inocula containing mixed microflora.
- Incubate at the optimal temperature required in aerobic conditions or else in an anaerobiosis jar, depending on the bacteria to be cultured.
- Determination of minimal inhibitory concentrations
- Add serial dilutions of the stock solution of antibiotic to test to tubes containing the medium.
- Inoculate with a defined quantity of the culture of the microorganism to test.
- Incubate for 24 hours at 37°C.
RESULTS
- Growth is determined by turbidity resulting from microbial division.
- The last tube with no microbial development contains antibiotic at a concentration which is the minimal inhibitory concentration. Multiplying this concentration by the corresponding dilution factor furnishes the concentration of active antibiotic
BacterLab ensures the quality of each product batch by testing with ATCC reference strains:
| Reference strains | Incubation conditions | Expected result |
| Escherichia coli ATCC 25922 | 35 – 37oC for 18 – 24 hours | Good growth |
| Pseudomonas aeruginosa ATCC 27853 | Good growth | |
| Enterococcus faecalis ATCC 29212 | Good growth | |
| Staphylococcus aureus ATCC 25923 | Good growth |
For 1 liter of medium
| Acid hydrolysate of casein | 17,5 g |
| Beef infusion | 2,0 g |
| Soluble starch | 1,5 g |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2.
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Mueller Hinton Broth (MHB) | 08026 | 50 tubes/ box or as per customer request. |
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