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INTENDED USE
- BacterTube™ Peptone Water is used for the culture of microorganisms with no particular growth requirements. This media is specifically used for the Mackenzie test for the identification of Escherichia coli via indole production.
PRINCIPLES
- Under aerobic conditions, Escherichia coli degrade tryptophan to indole through the action of a tryptophanase. The indole that is produced is detected using Kovacs reagent.
- Transfer a loop of enrichment broth to a pre-heated tube (44°C) of Peptone broth.
- Incubate at 44 ± 1 °C for 48 hours.
- Add 0.5 mL of indole reagent and mix well.
- Observe the tube after 1 minute.
RESULTS
- Based on the turbidity of the tube, the growth ability of strain can be determined.
- If perfoming the indole test, shake the tube well and observe the phenomenon on the surface of the medium:
- Positive Reaction: Amber to red color (indole activity detected).
- Negative Reaction: No color change (brownish remains).
BacterLab ensures the quality of each product batch by testing with ATCC reference strains.
| Reference strains | Incubation conditions | Expected results |
| Escherichia Coli ATCC 25922 | 18 – 24 hours, 35 – 37oC | Red medium: Indole positive |
| No bacterial | Unchanged medium: Indole negative |
For 1 liter of medium
| Peptone | 10,0 g |
| Sodium chloride | 5,0 g |
| Soluble starch | 1,5 g |
pH of the ready-to-use medium at 25°C: 7,3 ± 0,2
*Note: This ingredient is for reference only our company will formulate the ingredient according to requests or tender specifications.
| Product Name | Code | Packaging |
| BacterTube™ Peptone Water | 08040 | 50 tubes/ box or as per customer request. |
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