LabCell™ Ham’s F10

The medium is selected to support the growth of human diploid cells, white blood cells for chromosome analysis, primary cultures of mouse, rabbit and chicken tissues.
Code: 13004
Packaging: 500mL/ bottle or as per customer request.
Storage Conditions: 2–8°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

LabCell™ Ham’s F10 is the medium of choice for supporting the growth of human diploid cells, white blood cells for chromosome analysis, primary cultures of mouse, rabbit and chicken tissues.
LabCell™ Ham’s F10 is free of proteins and growth factors so serum supplementation is recommended depending on the cell type and application.

Use depends on the user’s purpose

BacterLab performs quality control on each product batch.

Parameter	Standard	Result
Sterility	No bacterial growth after incubation at 35 – 37°C for 18 – 24 hours	Pass

For 1 liter of medium (reference)

	LabCell™ Ham’s F10	Ham’s F10.1 mg/l	Ham’s F10.2 mg/l	Ham’s F10.2 mg/l
1	Glycine	7,51	7,51	7,51
	L-Alanine	9	9	9
	L-Arginine Monohydrochloride	211	211	211
	L-Asparagine Monohydrate	15,01	15,01	15,01
	L-Aspartic Acid	13,3	13,3	13,3
	L-Cysteine Monohydrochloride Monohydrate	35	35	35
	L-Glutamic Acid	14,7	14,7	14,7
	L-Glutamine	146	146	/
	L-Histidine Monohydrochloride Monohydrate	21	21	21
	L-Isoleucine	2,6	2,6	2,6
	L-Leucine	13,1	13,1	13,1
	L-Lysine Monohydrochloride	29,3	29,3	29,3
	L-Methionine	4,48	4,48	4,48
	L-Phenylalanine	4,96	4,96	4,96
	L-Proline	11,5	11,5	11,5
	L-Serine	10,5	10,5	10,5
	L-Threonine	3,57	3,57	3,57
	L-Tryptophan	0,6	0,6	0,6
	L-Tyrosine Disodium Salt Dihydrate	2,61	2,61	2,61
	L-Valine	3,5	3,5	3,5

2	Calcium Chloride Dihydrate	44,1	44,1	44,1
	Cupric Sulfate Pentahydrate	0,0025	0,0025	0,0025
	Ferrous Sulfate Heptahydrate	0,834	0,834	0,834
	Magnesium Sulfate Anhydrous	74,64	74,64	74,64
	Potassium Chloride	285	285	285
	Sodium Phosphate Monobasic Anhydrous	83	83	83
	Sodium Bicarbonate	1200	1200	1200
	Sodium Chloride	6800	7400	7400
	Sodium Phosphate Dibasic Anhydrous	153,7	153,7	153,7
	Zinc Sulfate Heptahydrate	0,0288	0,0288	0,0288

3	Choline Chloride	0,698	0,698	0,698
	D-Biotin	0,024	0,024	0,024
	D-Ca Pantothenate	0,715	0,715	0,715
	Folic Acid	1,32	1,32	1,32
	Myo-Inositol	0,541	0,541	0,541
	Nicotinamide	0,615	0,615	0,615
	Pyridoxine Hydrochloride	0,206	0,206	0,206
	Riboflavin	0,376	0,376	0,376
	Thiamine Hydrochloride	1	1	1
	Vitamin B12	1,36	1,36	1,36

4	D-Glucose Anhydrous	1100	1100	1100
	Hepes Free Acid	5958	/	/
	Hypoxanthine	4,08	4,08	4,08
	Phenol Red Solution Salt	1,3	1,3	1,3
	Sodium Pyruvate	110	110	110
	Thioctic Acid	0,21	0,21	0,21
	Thymidine	0,73	0,73	0,73