SpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL

Microbial Sampling Tool for surface sampling, using Cellulose sponge pre-moistened with Buffered Peptone Water containing neutralizers, used for monitoring surfaces and verifying sterilization and disinfection effectiveness.
Code: 4107026
Packaging:100 pieces/box or customer request.
Storage Conditions: 4 – 25°C.
Transportation: Ambient temperature.
Shelf Life: 24 months from the date of manufacture.

INTENDED USE

SpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL is intended for microbiological monitoring of surfaces and environmental objects, as well as for confirming and evaluating the effectiveness of sterilization and disinfection processes. It is suitable for use in medical, pharmaceutical, cosmetic, food, and other hygiene-sensitive industries.

PRINCIPLES

SpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL utilizes a cellulose sponge, combined with 10 mL of Buffered Peptone Water containing neutralizer to collect microorganisms from surfaces. The Buffered Peptone Water with neutralizing agents effectively neutralizes residual disinfectants and sanitizers on the sampled surfaces, supporting the recovery and preservation of microorganisms during sample collection, transport, and analysis. The sponge material and moisture content ensure optimal microbial viability and stability.

Inspect the SpongesCollect™ product to ensure there is no damage or contamination during storage.
Write sample information on the provided label attached to the product packaging.
Tear the bag along the pre-marked perforated line.
Push the sponge near the edge of the bag so that part of the sponge is exposed for easy handling. Hold the sponge from the outside of the bag and carefully remove it directly by hand, making sure not to touch the inside surface of the bag to avoid contamination.
Rub the SpongesCollect™ sponge on the area to be sampled.
After sampling, return the sponge to the bag. Ensure the sponge does not fall out and avoid direct contact with the outer surface of the packaging.
Use the provided tie to seal the SpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag.
The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.

Growth Promotion Test: ≤ 10³ CFU/mL microorganisms
Control strain	Incubation Conditions	Specifications
S. typhimurium ATCC 14028	18 – 24 h at 35 – 37°C	Good growth
S.aureus ATCC 25923	18 – 24 h at 35 – 37°C	Good growth
E. coli ATCC 25922	18 – 24 h at 35 – 37°C	Good growth
Sterility control	No bacterial growth after incubation at 35 – 37°C for 72 hrs
Appearance	The solution contains a homogeneous sponges, without any stratification, light yellow, clear.

Bacteriological peptone……………………………………… 10,0 g

Sodium Choloride……………………………………………… 5,0 g

Sodium phosphate dibasic 12-hydrate………………….. 9,0 g

Potassium phosphate monobasic anhydrous………….. 1,5 g

Tween 80 (Polysorbate 80)…………………………………. 3 mL

Histidine………………………………………………………….. 0,1 g

Sodium thiosulfate…………………………………………….. 0,8 g

Soy lecithin………………………………………………………. 0,3 g

pH of the ready-to-use medium at 25°C: 7,0 ± 0,2

* Adjusted and /or supplemented as required to meet performance criteria

Product Name	Code	Packaging
SpongesCollect™ Cellulose Buffered Peptone Water with neutralizers, 10 mL	4107026	100 pieces/box or customer request.