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SpongesCollect™ Polyurethane D/E Neutralizing Broth, 10 mL
- Microbial Sampling Tool for surface sampling, using a polyurethane sponge moistened with D/E Neutralizing Broth to neutralize the effects of residual disinfectants and protect microorganisms during transport and analysis.
- Code: 4107005
- Packaging: 100 pieces/box or customer request.
- Storage Conditions: 4 – 25°C.
- Transportation: Ambient temperature.
- Shelf Life: 24 months from the date of manufacture.
SpongesCollect™ Polyurethane D/E Neutralizing Broth, 10mL is designed for microbiological sampling from surfaces in food processing, healthcare, and pharmaceutical environments. The primary purpose of this product is to support the collection of microbial samples from large, easily accessible surfaces and surrounding areas within food production facilities. D/E Neutralizing Broth helps neutralize the effects of disinfectants and protects microorganisms throughout transport and analysis.
SpongesCollect™ Polyurethane D/E Neutralizing Broth, 10mL uses a biocide-free polyurethane sponge combined with 10 mL of D/E Neutralizing Broth to collect microorganisms from surfaces. D/E Neutralizing Broth neutralizes the effects of disinfectants such as chlorine, quaternary ammonium compounds, iodine, phenolic compounds, mercury, formaldehyde, and glutaraldehyde, protecting microorganisms from their harmful effects. This medium provides the necessary nutrients to recover and promote microorganism growth, while maintaining their viability during transport. D/E Neutralizing Broth not only neutralizes disinfectants but also preserves the integrity of microorganisms, ensuring accurate analysis results. Bromocresol purple is included in the medium as a pH indicator, allowing monitoring of the neutralization process.
- Inspect the SpongesCollect™ product to ensure there is no damage or contamination during storage.
- Write sample information on the provided label attached to the product packaging.
- Tear the bag along the pre-marked perforated line.
- Push the sponge near the edge of the bag so that part of the sponge is exposed for easy handling. Hold the sponge from the outside of the bag and carefully remove it directly by hand, making sure not to touch the inside surface of the bag to avoid contamination.
- Immerse the sponge into a sterile buffer/culture medium as required (if necessary).
- Rub the SpongesCollect™ sponge on the area to be sampled.
- After sampling, return the sponge to the bag. Ensure the sponge does not fall out and avoid direct contact with the outer surface of the packaging.
- Use the provided tie to seal the SpongesCollect™ bag by rolling the top of the bag and tightly twisting the tie inside the bag.
- The sample must be sent to the laboratory as soon as possible to prevent changes in microbial characteristics. The transportation process should comply with sample storage regulations.
| Growth Promotion Test: ≤ 103 CFU/mL microorganisms | ||
| Control strain | Incubation Conditions | Specifications |
| E. coli ATCC 25922 | 24 – 48 h at 35 – 37°C | Good growth |
| S. aureus ATCC 25923 | 24 – 48 h at 35 – 37°C | Good growth |
| S. typhimurium ATCC 14028 | 24 – 48 h at 35 – 37°C | Good growth |
| Sterility control | No bacterial growth after incubation at 35 – 37°C for 72 hrs | |
| Appearance | The solution contains a homogeneous sponges, without any stratification, purple, cloudy. | |
Dextrose………………………………………………………….. 10,0 g
Sodium bisulfite……………………………………………….. 2,5 g
Lecithin…………………………………………………………… 7,0 g
Yeast extract……………………………………………………. 2,5 g
Sodium thiosulfate……………………………………………. 6,0 g
Sodium thioglycollate……………………………………….. 1,0 g
Casein peptone…………………………………………………. 5,0 g
Brom cresol purple……………………………………………. 0,02 g
Polysorbate 80 (Tween 80)…………………………………. 5,0 g
pH of the ready-to-use medium at 25°C: 7,6 ± 0,2
* Adjusted and /or supplemented as required to meet performance criteria
| Product Name | Code | Packaging |
| SpongesCollect™ Polyurethane D/E Neutralizing Broth, 10 mL | 4107005 | 100 pieces/box or customer request. |
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